- ￥1350 - 3000
- Proteintech
- 10929-2-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human AMPK alpha protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
AMPK Alpha
- 抗体英文名：
AMPK Alpha Polyclonal antibody
- 抗体名：
AMPK Alpha Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells, J774A.1 cells, RAW 264.7 cells, PC-12 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10929-2-AP targets AMPK Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, zebrafish, goat, fish, carp |
| 免疫原 |
CatNo: Ag1367 Product name: Recombinant human AMPK alpha protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC012622 Sequence: MATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRDCNIIRILTSQFTNYQH |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| 别名 | AMPK Alpha 1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1, ACACA kinase |
| 计算分子量 | 63 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC012622 |
| 基因名称 | AMPK Alpha 1 |
| Gene ID (NCBI) | 5562 |
| RRID | AB_2169568 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13131 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nature Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.Authors - Xiao-Yi Lu |
| mouse | WB | Adv Sci (Weinh) Mitigating Doxorubicin-Induced Cardiotoxicity and Enhancing Anti-Tumor Efficacy with a Metformin-Integrated Self-Assembled NanomedicineAuthors - Jiaxin Huang |
| human | WB | Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagyAuthors - Kui Wang |
| human | WB,IP | Autophagy The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.Authors - Yongquan Hu
|
| rat,human | WB | J Adv Res A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial functionAuthors - Ximing Yang |
| WB | Autophagy A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector.Authors - Qifu Liang |
；加入羊抗人α2M多克隆抗体，37~C 2h，洗涤；加入HRP标记的兔抗羊IsC，孵育后加入底物呈色。终止反应后在酶标仪上读取492nm波长的吸光度值。按常规绘制标准曲线，算出待测血a2M含量。 3．正常值范围与临床意义 免疫扩散法测得正常男性血清a2M为1．5～3．5z／L，女性为1．35～4．2g／L。2—4岁儿童血清a2M约为成人的2～3倍。更年期妇女血清e2M含量也较高。ELBA法检测正常人尿液中Q，M含量均低于0．026mg／L
密切相关。本实验采用双抗体夹心法测定原发性肝癌患者血清sIL-2R水平。(夹心法原理参见实验五免疫酶技术) 即： 抗体-——待检血清——抗体——酶标抗抗体——显色 实验用品 1.材料、试剂：待测人血清、IL-2Rα阳性、阴性参考血清、IL-2Rα单抗、兔抗IL-2Rα多克隆抗体、酶结合物(HRP-抗兔IgG)、显色A液、B液、PBS 洗涤液、终止液。 2.器具：吸管、滴管、试管、48孔聚苯乙烯反应板、50&mu
相关。本实验采用双抗体夹心法测定原发性肝癌患者血清sIL-2R水平。（夹心法原理参见实验五免疫酶技术） 即： 抗体-——待检血清——抗体——酶标抗抗体——显色 实验用品 1．材料、 试剂 ：待测人血清、IL-2Rα阳性、阴性参考血清、IL-2Rα单抗、兔抗IL-2Rα多克隆抗体、酶结合物（HRP-抗兔IgG）、显色A液、B液、PBS洗涤液、终止液。 2．器具：吸管、滴管、 试管
技术资料暂无技术资料 索取技术资料
