- ￥980 - 6000
- Proteintech
- 10205-2-AP
- 2025年10月13日
- WB, IHC, IF-P, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PCNA protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IP, ELISA
- 靶点：
PCNA
- 抗体英文名：
PCNA Polyclonal antibody
- 抗体名：
PCNA Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, HEK293 cells, BALB/3T3 clone A31 cells, mouse spleen tissue, HeLa cells, Jurkat cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, rat spleen tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human breast cancer tissue, human malignant melanoma tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue, human breast cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10205-2-AP targets PCNA in WB, IHC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, Cell treatment |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken, goat, sheep, fish, ducks, medaka embryos |
| 免疫原 |
CatNo: Ag0277 Product name: Recombinant human PCNA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-256 aa of BC000491 Sequence: QGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proliferating cell nuclear antigen |
| 别名 | Cyclin, proliferating cell nuclear antigen |
| 计算分子量 | 29 kDa/31 kDa |
| 观测分子量 | 36-38 kDa |
| GenBank蛋白编号 | BC000491 |
| 基因名称 | PCNA |
| Gene ID (NCBI) | 5111 |
| RRID | AB_2160330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
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文献和实验| Species | Application | Title |
|---|---|---|
| WB | J Hematol Oncol METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translationAuthors - Meilin Xue | |
| mouse,human | WB | Protein Cell NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferationAuthors - Yirui Cheng |
| human | WB | Mil Med Res Aspartoacylase suppresses prostate cancer progression by blocking LYN activationAuthors - Hong Weng |
| mouse | IF | Brain Behav Immun An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling.Authors - Fangfang Qi |
| human | WB | Nat Commun The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancerAuthors - Wei Zhou |
| rabbit | IF | Nat Commun Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstructionAuthors - Yujie Hua |
【求助】免疫组化, FISH, CGH, LOH, MSI, 克隆性重排等的应用范围及应用”盲点”
性 （2）对疾病的预后和治疗的意义 （3）癌基因蛋白的应用 （4）对肿瘤增生程度的评价 ki67 PCNA （5）微小病灶的发现 微小癌 微小病灶（如羊水栓塞） （6）在肿瘤分期上的意义 （7）指导肿瘤治疗 （8）免疫性疾病的辅助诊断 （9）病原微生物的检测 应用“盲点”：由于抗体稀释度、多克隆抗体特异性问题、试剂盒选择、操作中时间温度染色过程等因素
是由于切片的原因所致。但是，抗原的表达，在原来的表达上就有核、浆、膜之分。有的抗原可在一个部位表达，有的抗原 则可在两个 部位表达 ，例如PCNA，它可以在细胞核表达，也可在细胞浆表达 。有的抗原，可表现为双向表达，如上皮(样)肿瘤间皮肿瘤和滑膜内瘤等，用角蛋白标记时，可有阳性表达，用波形蛋白标记时，也可有阳性表达。对于上述的许多现象，在观察检测时，就要特别地小心，不断地积累经验，以便于更好地鉴别各种抗原的所在部位。某些抗原不是在任何时候能表达出来的它们 需要一定的时间和条件。有人根据肿瘤细胞增生的能力
、浆、膜之分。有的抗原可在一个部位表达，有的抗原 则可在两个 部位表达 ，例如PCNA，它可以在细胞核表达，也可在细胞浆表达 。有的抗原，可表现为双向表达，如上皮(样)肿瘤间皮肿瘤和滑膜内瘤等，用角蛋白标记时，可有阳性表达，用波形蛋白标记时，也可有阳性表达。对于上述的许多现象，在观察检测时，就要特别地小心，不断地积累经验，以便于更好地鉴别各种抗原的所在部位。某些抗原不是在任何时候能表达出来的它们 需要一定的时间和条件。有人根据肿瘤细胞增生的能力将其分为三个阶段： 1.进入
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