- ￥1350 - 3000
- Proteintech
- 10084-2-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CD71 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
CD71
- 抗体英文名：
CD71 Polyclonal antibody
- 抗体名：
CD71 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HT-1080 cells, LoVo cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10084-2-AP targets CD71 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, Cell treatment |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, monkey, chicken, bovine, duck |
| 免疫原 |
CatNo: Ag0123 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Domain: of Sequence: |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transferrin receptor (p90, CD71) |
| 别名 | p90, sTfR, T9, TFR, TFR1 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC001188 |
| 基因名称 | CD71 |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_2240403 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02786 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.Authors - Jia-Yuan Zhang |
| human | IHC | Acta Neuropathol Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.Authors - Till S Zimmer |
| WB,IHC | Cell Death Differ Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasisAuthors - Qing-Xin Ji | |
| WB,IF | J Nanobiotechnology Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reactionAuthors - Xue Li | |
| human | WB | Int J Biol Sci PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1Authors - Bingyan Xue |
| WB | Acta Biomater Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytesAuthors - Wenqiang Qian |
and run them immediately after staining is accomplished 因此，如果你没有用PBS洗的话，可以用paraformaldehyde固定，放置48小时。洗了后应该尽快检测。如果做好不能及时检测，我们一般是加入等量的2%的多聚甲醛固定，4度冰箱避光贮存。一般过夜没有问题，第二天同样PBS 2ml 洗涤细胞一次，上机检测。 18、如何分选原始红细胞？ CD71（转铁蛋白受体）--幼稚细胞的标记 CD235（GPA）--红细胞的标记（小鼠有Ter119
，放置48小时。洗了后应该尽快检测。如果做好不能及时检测，我们一般是加入等量的2%的多聚甲醛固定，4度冰箱避光贮存。一般过夜没有问题，第二天同样PBS 2ml 洗涤细胞一次，上机检测。18、如何分选原始红细胞？CD71（转铁蛋白受体）--幼稚细胞的标记CD235（GPA）--红细胞的标记（小鼠有Ter119）双阳性细胞即为幼稚红细胞，但无法区分原始及中晚幼红.19、请问流式检测膜蛋白表达的变化用多克隆抗体出结果的机会大吗？流式的抗体和WB的抗体是不同的（有部分可以通用），多抗一般是大的变性蛋白为抗原
甲醛固定，4度冰箱避光贮存。一般过夜没有问题，第二天同样PBS 2ml 洗涤细胞一次，上机检测。 18、如何分选原始红细胞？ CD71（转铁蛋白受体）--幼稚细胞的标记 CD235（GPA）--红细胞的标记（小鼠有Ter119） 双阳性细胞即为幼稚红细胞，但无法区分原始及中晚幼红. 19、请问流式检测膜蛋白表达的变化用多克隆抗体出结果的机会大吗？ 流式的抗体和WB的抗体是不同的（有部分可以通用），多抗一般是大的变性蛋白为抗原制备的，而WB的中被检测蛋白就是变性
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