- ￥1350 - 3000
- Proteintech
- 10048-2-Ig
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RB1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
RB1
- 抗体英文名：
RB1 Polyclonal antibody
- 抗体名：
RB1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, Calyculin A treated Jurkat cells, MCF-7 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10048-2-Ig targets RB1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, zebrafish |
| 免疫原 |
CatNo: Ag0006 Product name: Recombinant human RB1 protein Source: e coli.-derived, Unknow Tag: N/A Domain: Gst-Rb1-928 of BC040540 Sequence: |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoblastoma 1 |
| 别名 | pRb, pp110, p110-RB1 , p110-RB1, p105-Rb |
| 计算分子量 | 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC040540 |
| 基因名称 | RB1 |
| Gene ID (NCBI) | 5925 |
| RRID | AB_2177320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| IHC | Nat Genet Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.Authors - Wang Guocan G | |
| human | IHC | Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.Authors - Yuhong Luo |
| human | WB, IHC | J Clin Invest TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.Authors - Zheng Hu |
| human | WB | Nat Commun Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.Authors - Kai Yuan |
| human | WB | Front Med Dronedarone inhibits the proliferation of esophageal squamous cell carcinoma through the CDK4/CDK6-RB1 axis in vitro and in vivoAuthors - Bo Li |
| human | WB | Theranostics Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.Authors - Shuai Huang |
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该法可用于分离提取人、兔、小鼠血清中的Ig及杂交瘤诱生的腹水和培养上清液中McAb。 一、材料和试剂 1. 正辛酸。 2. 60mmol/L，pH4.0醋酸缓冲液，PBS。 3. 透析袋。 4. 高速低温离心机。 二、操作步骤 1. 将待提取样品用60mmol/L，pH4.0醋酸缓冲液稀释3～4倍，调pH至4.5，对含脂质较高的标本可采用二氧化硅粉或玻璃纤维吸附法除去脂质。 2. 在室温下边搅拌边缓慢加入正辛酸，按每ml样品中加25μl，若样品体积
多克隆抗体技术（Polyclone antibodies preparation technique）
隆抗体混杂在一起就是多克隆抗体，机体内所产生的抗体就是多克隆抗体；除了抗原决定簇的多样性以外，同样一类抗原决定簇，也可刺激机体产生IgG、IgM、IgA、IgE和IgD等五类抗体。 （二）免疫 动物 供免疫用的动物主要是哺乳动物和禽类，常选择家兔、绵羊、山羊、马、骡和豚鼠及小鼠等。动物的选择常根据抗体的用途和量来决定，也与抗原的性质有关。如要获得大量的抗体，多采用大动物；如要是获得直接标记诊断的抗体，则直接采用本动物；如要获得间接的标记诊断用抗体，则必须用异源动物制备抗体；如果难以
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