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- 2025年12月06日
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- 详细信息
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
NEBNext® Ultra™ II Ligation Module 收藏
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors
compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.
The NEBNext Ultra II Ligation Module is Designed for use with the Following:
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535)
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Ultra II Ligation Master Mix | -20 | |
| NEBNext Ligation Enhancer | -20 |
Description
The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors
compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.
The NEBNext Ultra II Ligation Module is Designed for use with the Following:
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535)
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Ultra II Ligation Master Mix | -20 | |
| NEBNext Ligation Enhancer | -20 |
Properties and Usage
Storage Temperature
-20°C
上海淳麦生物科技有限公司专注于感受态细胞、质粒、载体、农杆菌、酵母等生命科学领域的产品研发和技术服务支持。
上海淳麦生物科技有限公司在感受态细胞的研发方面，实力雄厚，拥有了克隆、表达、酵母和农杆菌4大类，共计百余种感受态细胞。在电击感受态方面，拥有了大肠杆菌和农杆菌电击感受态种类，极大提高了客户在转基因以及文库构建方面的成功率。
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