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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3) 收藏
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
The NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) contains adaptors and primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.
Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (Index Primers Set 3)
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Universal PCR Primer for Illumina | -20 | |
| NEBNext Adaptor for Illumina | -20 | |
| USER Enzyme | -20 | |
| NEBNext Index 17 Primer for Illumina | -20 | |
| NEBNext Index 24 Primer for Illumina | -20 | |
| NEBNext Index 26 Primer for Illumina | -20 | |
| NEBNext Index 28 Primer for Illumina | -20 | |
| NEBNext Index 29 Primer for Illumina | -20 | |
| NEBNext Index 31 Primer for Illumina | -20 | |
| NEBNext Index 32 Primer for Illumina | -20 | |
| NEBNext Index 33 Primer for Illumina | -20 | |
| NEBNext Index 34 Primer for Illumina | -20 | |
| NEBNext Index 35 Primer for Illumina | -20 | |
| NEBNext Index 36 Primer for Illumina | -20 | |
| NEBNext Index 42 Primer for Illumina | -20 |
|
Description
The NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) contains adaptors and primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.
Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (Index Primers Set 3)
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Universal PCR Primer for Illumina | -20 | |
| NEBNext Adaptor for Illumina | -20 | |
| USER Enzyme | -20 | |
| NEBNext Index 17 Primer for Illumina | -20 | |
| NEBNext Index 24 Primer for Illumina | -20 | |
| NEBNext Index 26 Primer for Illumina | -20 | |
| NEBNext Index 28 Primer for Illumina | -20 | |
| NEBNext Index 29 Primer for Illumina | -20 | |
| NEBNext Index 31 Primer for Illumina | -20 | |
| NEBNext Index 32 Primer for Illumina | -20 | |
| NEBNext Index 33 Primer for Illumina | -20 | |
| NEBNext Index 34 Primer for Illumina | -20 | |
| NEBNext Index 35 Primer for Illumina | -20 | |
| NEBNext Index 36 Primer for Illumina | -20 | |
| NEBNext Index 42 Primer for Illumina | -20 |
|
Properties and Usage
Materials Required but not Supplied
Enzymes and buffers appropriate for DNA or ChIP Illumina library preparation. (DNA:
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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
reaction containing 10mM Tris-Hcl (pH 8.3), 50 mM KCl, 2 mM MgCl2 , 50µM dNTP, 0.5µM each primer, and 0.6 unit of Taq DNA polymerase (Cinagen, Tehran, IRAN). The primers used were 3-A (5'-TAGTCACTGGCAACAGTCTT-3') and 3-B (5'-AAAATCCCTGTTCCCACTCATAC¨C3
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Differential Display Technical notes
Optimal arbitrary primer length for Differential Display The concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to systematically
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