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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) 收藏
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation. Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a combination of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal. NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols
Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (96 Index Primers)
Functionally Validated: Each set of reagents is functionally validated together through construction and sequencing of genomic DNA libraries on the Illumina platform.
Lot Control: The lots provided in the NEBNext Multiplex Oligos for Illumina (96 Index Primers) are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext® Adaptor for Illumina® | -20 | |
| USER™ Enzyme | -20 | |
| NEBNext® Index/Universal Primer Mix (1-96) | -20 |
Description
Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation. Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a combination of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal. NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols
Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (96 Index Primers)
Functionally Validated: Each set of reagents is functionally validated together through construction and sequencing of genomic DNA libraries on the Illumina platform.
Lot Control: The lots provided in the NEBNext Multiplex Oligos for Illumina (96 Index Primers) are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext® Adaptor for Illumina® | -20 | |
| USER™ Enzyme | -20 | |
| NEBNext® Index/Universal Primer Mix (1-96) | -20 |
Properties and Usage
Materials Required but not Supplied
- Enzymes and buffers appropriate for DNA, ChIP or mRNA Library Preparation (NEB #E7645, E7595,E7420, E7530, E7370, E7445, E6040, E6110, E6240, E6056, E6000, E6200, E6100 )
- SPRIselect or AMPure® XP Beads (Beckman Coulter, Inc.)
- DNA LoBind® Tubes (Eppendorf®)/PCR Plates
- Magnetic Stand
- Bioanalyzer® (Agilent Technologies, Inc.)
- Freshly Prepared 80% Ethanol
Please Refer to the Kit Specific Protocol for using the NEBNext Multiplex Oligos for Illumina:
The following kits are designed for use with the NEBNext Multiplex Oligos for Illumina:
- #E7645 , NEBNext Ultra II DNA Library Prep Kit for Illumina
- #E7595 , NEBNext Ultra II Ligation Module
- #E7420 , NEBNext Ultra Directional RNA Library Prep Kit for Illumina
- #E7530 , NEBNext Ultra RNA Library Prep Kit for Illumina
- #E7370 , NEBNext Ultra DNA Library Prep Kit for Illumina
- #E7445 , NEBNext Ultra Ligation Module
- #E6040 , NEBNext DNA Library Prep Master Mix Set for Illumina
- #E6110 , NEBNext mRNA Library Prep Master Mix Set for Illumina
- #E6240 , NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
- #E6056 , NEBNext Quick Ligation Module
- #E6000 , NEBNext DNA Library Prep Reagent Set for Illumina
- #E6200 , NEBNext ChIP-Seq Library Prep Reagent Set for Illumina
- #E6100 , NEBNext mRNA Library Prep Reagent Set for Illumina
Troubleshooting
Troubleshooting
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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
reaction containing 10mM Tris-Hcl (pH 8.3), 50 mM KCl, 2 mM MgCl2 , 50µM dNTP, 0.5µM each primer, and 0.6 unit of Taq DNA polymerase (Cinagen, Tehran, IRAN). The primers used were 3-A (5'-TAGTCACTGGCAACAGTCTT-3') and 3-B (5'-AAAATCCCTGTTCCCACTCATAC¨C3
Differential Display Technical notes
Optimal arbitrary primer length for Differential Display The concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to systematically
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
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