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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
PageRuler Plus Prestained Protein Ladder, 2 x 250 uL.
- 供应商：
北京智杰方远科技
- 保存条件：
详见说明
- 规格：
2 X 250 UL
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
Bradford Protein Determination
of the specified compound before adding 200 uL of dye-reagent. According to Stoscheck, C. Increased uniformity in the response of the Coomassie blue protein assay to different proteins. Anal. Biochem. (1990) 184, 111-116.
Small scale His-Tag fusion protein purification under denaturative conditions
6 to 8M or Guanidine-HCl 6M (try 8M of Urea first, and if protein is soluble titer down in the next experiments till urea concentration till minimal urea is required for protein solubilization) 3 Sonicate on ice 3 x 20 seconds (depends
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