pAAV2-8腺相关病毒质粒

基本信息

别名:	pAAV-RC8
原核抗性:	氨苄青霉素Amp
克隆菌株:	大肠杆菌Stbl3
培养条件:	37℃

质粒简介

               pAAV2-8是一个腺相关病毒血清型质粒。

质粒图谱

质粒序列

LOCUS       Exported               21837 bp ds-DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..21837
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             1346..3211
                     /codon_start=1
                     /label=Rep
                     /translation="MPGFYEIVIKVPSDLDGHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     CDS             3228..5135
                     /codon_start=1
                     /label=Cap
                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGL
                     VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERL
                     QEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKK
                     GQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVGPNTMAAGGGAPMADNNEGADGV
                     GSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYSTP
                     WGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLT
                     STIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLE
                     YFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTA
                     NTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRN
                     SLANPGIAMATHKDDEERFFPSNGILILANKMLPETMRITAMSCSPA?KKSKPLTLWLQ
                     RNTVSWQITCSSKTRLLKLELSTARGPYPVWSGRTGTCTCRVPSGPRFLTRTATSTRLR
                     "
     repeat_region   19739..19841
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(20032..20620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(20772..21632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPATMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(21633..21737)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
       61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
      121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
      181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
      241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
      301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
      361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
      421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
      481 cctctagaac tagtggatcc gtccctcagg cctagaagta aaaaagggaa aaaagagtgt
      541 gtttgtcaaa ataggagaca ggtggtggca accaaggact tataggggac cttacatcta
      601 cagaccaaca gatgccccct taccatatac aggaagatat gacttaaatt gggataggtg
      661 ggtcacaatc aacggctata aagtgttata cagatccctc ccctcccctt tcgtgaaaga
      721 ctcgccagag ctagacctcc ttggtgtatg ctaactgaga agagaaagac gacatgaaac
      781 aacaggtaca tgattatatt tatctaggaa caggaatgca cttttgggga aaggttttcc
      841 ataccaagga aggggcagtg gctggactga tagaacatta ttctgcaaaa acttatggta
      901 tgagttatta tgattagcct ttatttgccc aaccttgcgg ttcccagggt ttaaataagt
      961 ttatggttac aaactgttct taaaacgagg atgtgagaca agtggtttcc tgacttggtt
     1021 tggtaatcaa atgttctgat ctgagctctt agtgttctat tttcctatgt tcttttggaa
     1081 tctatccaag tcttatgtaa atgcttatgt aaaccataat ataaaagagt gctgattttt
     1141 tgagtaaact tgcaacagtc ctaacattct tctctcgtgt gtttgtgtct gttcgccatc
     1201 ccgtctccgc tcgtcactta tccttcactt ttcagagggt ccccccgcag atcccggtca
     1261 ccctcaggtc gggacctgca gaagacgccc gagtgagcac gcagggtctc cattttgaag
     1321 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc
     1381 cagcgacctt gacgggcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga
     1441 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc
     1501 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa
     1561 ggccccggag gcccttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca
     1621 cgtgctcgtg gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat
     1681 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt
     1741 cgcggtcaca aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta
     1801 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat
     1861 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca
     1921 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga
     1981 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt
     2041 ggacaagggg attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc
     2101 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa
     2161 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga
     2221 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc
     2281 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct
     2341 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc
     2401 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa
     2461 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc
     2521 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga
     2581 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc
     2641 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg
     2701 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg
     2761 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa
     2821 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt
     2881 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa
     2941 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag
     3001 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt
     3061 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta
     3121 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt
     3181 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg
     3241 gttatcttcc agattggctc gaggacaacc tctctgaggg cattcgcgag tggtgggcgc
     3301 tgaaacctgg agccccgaag cccaaagcca accagcaaaa gcaggacgac ggccggggtc
     3361 tggtgcttcc tggctacaag tacctcggac ccttcaacgg actcgacaag ggggagcccg
     3421 tcaacgcggc ggacgcagcg gccctcgagc acgacaaggc ctacgaccag cagctgcagg
     3481 cgggtgacaa tccgtacctg cggtataacc acgccgacgc cgagtttcag gagcgtctgc
     3541 aagaagatac gtcttttggg ggcaacctcg ggcgagcagt cttccaggcc aagaagcggg
     3601 ttctcgaacc tctcggtctg gttgaggaag gcgctaagac ggctcctgga aagaagagac
     3661 cggtagagcc atcaccccag cgttctccag actcctctac gggcatcggc aagaaaggcc
     3721 aacagcccgc cagaaaaaga ctcaattttg gtcagactgg cgactcagag tcagttccag
     3781 accctcaacc tctcggagaa cctccagcag cgccctctgg tgtgggacct aatacaatgg
     3841 ctgcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga gtgggtagtt
     3901 cctcgggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc accaccagca
     3961 cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc tccaacggga
     4021 catcgggagg agccaccaac gacaacacct acttcggcta cagcaccccc tgggggtatt
     4081 ttgactttaa cagattccac tgccactttt caccacgtga ctggcagcga ctcatcaaca
     4141 acaactgggg attccggccc aagagactca gcttcaagct cttcaacatc caggtcaagg
     4201 aggtcacgca gaatgaaggc accaagacca tcgccaataa cctcaccagc accatccagg
     4261 tgtttacgga ctcggagtac cagctgccgt acgttctcgg ctctgcccac cagggctgcc
     4321 tgcctccgtt cccggcggac gtgttcatga ttccccagta cggctaccta acactcaaca
     4381 acggtagtca ggccgtggga cgctcctcct tctactgcct ggaatacttt ccttcgcaga
     4441 tgctgagaac cggcaacaac ttccagttta cttacacctt cgaggacgtg cctttccaca
     4501 gcagctacgc ccacagccag agcttggacc ggctgatgaa tcctctgatt gaccagtacc
     4561 tgtactactt gtctcggact caaacaacag gaggcacggc aaatacgcag actctgggct
     4621 tcagccaagg tgggcctaat acaatggcca atcaggcaaa gaactggctg ccaggaccct
     4681 gttaccgcca acaacgcgtc tcaacgacaa ccgggcaaaa caacaatagc aactttgcct
     4741 ggactgctgg gaccaaatac catctgaatg gaagaaattc attggctaat cctggcatcg
     4801 ctatggcaac acacaaagac gacgaggagc gtttttttcc cagtaacggg atcctgattt
     4861 tggcaaacaa aatgctgcca gagacaatgc ggattacagc gatgtcatgc tcaccagcga
     4921 gnaagaaatc aaaaccacta accctgtggc tacagaggaa tacggtatcg tggcagataa
     4981 cttgcagcag caaaacacgg ctcctcaaat tggaactgtc aacagccagg gggccttacc
     5041 cggtatggtc tggcagaacc gggacgtgta cctgcagggt cccatctggg ccaagattcc
     5101 tcacacggac ggcaacttcc acccgtctcc gctgatgggc ggctttggcc tgaaacatcc
     5161 tccgcctcag atcctgatca agaacacgcc tgtacctgcg gatcctccga ccaccttcaa
     5221 ccagtcaaag ctgaactctt tcatcacgca atacagcacc ggacaggtca gcgtggaaat
     5281 tgaatgggag ctgcagaagg aaaacagcaa gcgctggaac cccgagatcc agtacacctc
     5341 caactactac aaatctacaa gtgtggactt tgctgttaat acagaaggcg tgtactctga
     5401 accccgcccc attggcaccc gttacctcac ccgtaatctg taattgcctg ttaatcaata
     5461 aaccggttga ttcgtttcag ttgaactttg gtctctgcga agggcgaatt cgtttccatc
     5521 gatgcatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg ccccaggctt
     5581 cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct accggcactt
     5641 cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg gcggcggagt
     5701 ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc ctcatcggct
     5761 gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc acctgcgtga
     5821 gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg ttgatggtgt
     5881 aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc gagagctcgg
     5941 tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa gtccgcacca
     6001 ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg
     6061 tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg tagatgtacc
     6121 tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt
     6181 tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtcaggc
     6241 gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg ggcactcttc
     6301 cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt tcgagccccg
     6361 tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc caggtgtgcg
     6421 acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg gctgctgcgc
     6481 tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa gcgaaagcat
     6541 taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc gcgggacccc
     6601 cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc ccgtcatgca
     6661 agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc ttttcccaga
     6721 tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag caagagcagc
     6781 ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg acatccgcgg
     6841 ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg cactacctgg
     6901 acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag cggtacccaa
     6961 gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac ctgtttcgcg
     7021 accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca gggcgcgagc
     7081 tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag cccgacgcgc
     7141 gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta accgcatacg
     7201 agcagacggt gaaccagggc gatcgcaccc tttggcgcat cccattctcc agtaacttta
     7261 tgtccatggg cgcactcaca gacctgggcc aaaaccttct ctacgccaac tccgcccacg
     7321 cgctagacat gacttttgag gtggatccca tggacgagcc cacccttctt tatgttttgt
     7381 ttgaagtctt tgacgtggtc cgtgtgcacc ggccgcaccg cggcgtcatc gaaaccgtgt
     7441 acctgcgcac gcccttctcg gccggcaacg ccacaacata aagaagcaag caacatcaac
     7501 aacagctgcc gccatgggct ccagtgagca ggaactgaaa gccattgtca aagatcttgg
     7561 ttgtgggcca tattttttgg gcacctatga caagcgcttt ccaggctttg tttctccaca
     7621 caagctcgcc tgcgccatag tcaatacggc cggtcgcgag actgggggcg tacactggat
     7681 ggcctttgcc tggaacccgc actcaaaaac atgctacctc tttgagccct ttggcttttc
     7741 tgaccagcga ctcaagcagg tttaccagtt tgagtacgag tcactcctgc gccgtagcgc
     7801 cattgcttct tcccccgacc gctgtataac gctggaaaag tccacccaaa gcgtacaggg
     7861 gcccaactcg gccgcctgtg gactattctg ctgcatgttt ctccacgcct ttgccaactg
     7921 gccccaaact cccatggatc acaaccccac catgaacctt attaccgggg tacccaactc
     7981 catgctcaac agtccccagg tacagcccac cctgcgtcgc aaccaggaac agctctacag
     8041 cttcctggag cgccactcgc cctacttccg cagccacagt gcgcagatta ggagcgccac
     8101 ttctttttgt cacttgaaaa acatgtaaaa ataatgtact agagacactt tcaataaagg
     8161 caaatgcttt tatttgtaca ctctcgggtg attatttacc cccacccttg ccgtctgcgc
     8221 cgtttaaaaa tcaaaggggt tctgccgcgc atcgctatgc gccactggca gggacacgtt
     8281 gcgatactgg tgtttagtgc tccacttaaa ctcaggcaca accatccgcg gcagctcggt
     8341 gaagttttca ctccacaggc tgcgcaccat caccaacgcg tttagcaggt cgggcgccga
     8401 tatcttgaag tcgcagttgg ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt
     8461 gcagcactgg aacactatca gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga
     8521 gatcagatcc gcgtccaggt cctccgcgtt gctcagggcg aacggagtca actttggtag
     8581 ctgccttccc aaaaagggcg cgtgcccagg ctttgagttg cactcgcacc gtagtggcat
     8641 caaaaggtga ccgtgcccgg tctgggcgtt aggatacagc gcctgcataa aagccttgat
     8701 ctgcttaaaa gccacctgag cctttgcgcc ttcagagaag aacatgccgc aagacttgcc
     8761 ggaaaactga ttggccggac aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga
     8821 gatctgcacc acatttcggc cccaccggtt cttcacgatc ttggccttgc tagactgctc
     8881 cttcagcgcg cgctgcccgt tttcgctcgt cacatccatt tcaatcacgt gctccttatt
     8941 tatcataatg cttccgtgta gacacttaag ctcgccttcg atctcagcgc agcggtgcag
     9001 ccacaacgcg cagcccgtgg gctcgtgatg cttgtaggtc acctctgcaa acgactgcag
     9061 gtacgcctgc aggaatcgcc ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag
     9121 ctgcaacccg cggtgctcct cgttcagcca ggtcttgcat acggccgcca gagcttccac
     9181 ttggtcaggc agtagtttga agttcgcctt tagatcgtta tccacgtggt acttgtccat
     9241 cagcgcgcgc gcagcctcca tgcccttctc ccacgcagac acgatcggca cactcagcgg
     9301 gttcatcacc gtaatttcac tttccgcttc gctgggctct tcctcttcct cttgcgtccg
     9361 cataccacgc gccactgggt cgtcttcatt cagccgccgc actgtgcgct tacctccttt
     9421 gccatgcttg attagcaccg gtgggttgct gaaacccacc atttgtagcg ccacatcttc
     9481 tctttcttcc tcgctgtcca cgattacctc tggtgatggc gggcgctcgg gcttgggaga
     9541 agggcgcttc tttttcttct tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg
     9601 cgggctgggt gtgcgcggca ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc
     9661 gatacgccgc ctcatccgct tttttggggg cgcccgggga ggcggcggcg acggggacgg
     9721 ggacgacacg tcctccatgg ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt
     9781 ggtttcgcgc tgctcctctt cccgactggc catttccttc tcctataggc agaaaaagat
     9841 catggagtca gtcgagaaga aggacagcct aaccgccccc tctgagttcg ccaccaccgc
     9901 ctccaccgat gccgccaacg cgcctaccac cttccccgtc gaggcacccc cgcttgagga
     9961 ggaggaagtg attatcgagc aggacccagg ttttgtaagc gaagacgacg aggaccgctc
    10021 agtaccaaca gaggataaaa agcaagacca ggacaacgca gaggcaaacg aggaacaagt
    10081 cgggcggggg gacgaaaggc atggcgacta cctagatgtg ggagacgacg tgctgttgaa
    10141 gcatctgcag cgccagtgcg ccattatctg cgacgcgttg caagagcgca gcgatgtgcc
    10201 cctcgccata gcggatgtca gccttgccta cgaacgccac ctattctcac cgcgcgtacc
    10261 ccccaaacgc caagaaaacg gcacatgcga gcccaacccg cgcctcaact tctaccccgt
    10321 atttgccgtg ccagaggtgc ttgccaccta tcacatcttt ttccaaaact gcaagatacc
    10381 cctatcctgc cgtgccaacc gcagccgagc ggacaagcag ctggccttgc ggcagggcgc
    10441 tgtcatacct gatatcgcct cgctcaacga agtgccaaaa atctttgagg gtcttggacg
    10501 cgacgagaag cgcgcggcaa acgctctgca acaggaaaac agcgaaaatg aaagtcactc
    10561 tggagtgttg gtggaactcg agggtgacaa cgcgcgccta gccgtactaa aacgcagcat
    10621 cgaggtcacc cactttgcct acccggcact taacctaccc cccaaggtca tgagcacagt
    10681 catgagtgag ctgatcgtgc gccgtgcgca gcccctggag agggatgcaa atttgcaaga
    10741 acaaacagag gagggcctac ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac
    10801 gcgcgagcct gccgacttgg aggagcgacg caaactaatg atggccgcag tgctcgttac
    10861 cgtggagctt gagtgcatgc agcggttctt tgctgacccg gagatgcagc gcaagctaga
    10921 ggaaacattg cactacacct ttcgacaggg ctacgtacgc caggcctgca agatctccaa
    10981 cgtggagctc tgcaacctgg tctcctacct tggaattttg cacgaaaacc gccttgggca
    11041 aaacgtgctt cattccacgc tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt
    11101 ttacttattt ctatgctaca cctggcagac ggccatgggc gtttggcagc agtgcttgga
    11161 ggagtgcaac ctcaaggagc tgcagaaact gctaaagcaa aacttgaagg acctatggac
    11221 ggccttcaac gagcgctccg tggccgcgca cctggcggac atcattttcc ccgaacgcct
    11281 gcttaaaacc ctgcaacagg gtctgccaga cttcaccagt caaagcatgt tgcagaactt
    11341 taggaacttt atcctagagc gctcaggaat cttgcccgcc acctgctgtg cacttcctag
    11401 cgactttgtg cccattaagt accgcgaatg ccctccgccg ctttggggcc actgctacct
    11461 tctgcagcta gccaactacc ttgcctacca ctctgacata atggaagacg tgagcggtga
    11521 cggtctactg gagtgtcact gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg
    11581 caattcgcag ctgcttaacg aaagtcaaat tatcggtacc tttgagctgc agggtccctc
    11641 gcctgacgaa aagtccgcgg ctccggggtt gaaactcact ccggggctgt ggacgtcggc
    11701 ttaccttcgc aaatttgtac ctgaggacta ccacgcccac gagattaggt tctacgaaga
    11761 ccaatcccgc ccgccaaatg cggagcttac cgcctgcgtc attacccagg gccacattct
    11821 tggccaattg caagccatca acaaagcccg ccaagagttt ctgctacgaa agggacgggg
    11881 ggtttacttg gacccccagt ccggcgagga gctcaaccca atccccccgc cgccgcagcc
    11941 ctatcagcag cagccgcggg cccttgcttc ccaggatggc acccaaaaag aagctgcagc
    12001 tgccgccgcc acccacggac gaggaggaat actgggacag tcaggcagag gaggttttgg
    12061 acgaggagga ggaggacatg atggaagact gggagagcct agacgaggaa gcttccgagg
    12121 tcgaagaggt gtcagacgaa acaccgtcac cctcggtcgc attcccctcg ccggcgcccc
    12181 agaaatcggc aaccggttcc agcatggcta caacctccgc tcctcaggcg ccgccggcac
    12241 tgcccgttcg ccgacccaac cgtagatggg acaccactgg aaccagggcc ggtaagtcca
    12301 agcagccgcc gccgttagcc caagagcaac aacagcgcca aggctaccgc tcatggcgcg
    12361 ggcacaagaa cgccatagtt gcttgcttgc aagactgtgg gggcaacatc tccttcgccc
    12421 gccgctttct tctctaccat cacggcgtgg ccttcccccg taacatcctg cattactacc
    12481 gtcatctcta cagcccatac tgcaccggcg gcagcggcag cggcagcaac agcagcggcc
    12541 acacagaagc aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg
    12601 gcggcagcag caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc
    12661 gagcttagaa acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa
    12721 gaacaagagc tgaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat
    12781 cacaaaagcg aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa
    12841 tactgcgcgc tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa
    12901 ctacgtcatc tccagcggcc acacccggcg ccagcacctg tcgtcagcgc cattatgagc
    12961 aaggaaattc ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga
    13021 gctgcccaag actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc
    13081 cgggtcaacg gaatccgcgc ccaccgaaac cgaattctct tggaacaggc ggctattacc
    13141 accacacctc gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa
    13201 agtcccgctc ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact
    13261 aactcagggg cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt
    13321 ataactcacc tgacaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc
    13381 tcgcttggtc tccgtccgga cgggacattt cagatcggcg gcgccggccg ctcttcattc
    13441 acgcctcgtc aggcaatcct aactctgcag acctcgtcct ctgagccgcg ctctggaggc
    13501 attggaactc tgcaatttat tgaggagttt gtgccatcgg tctactttaa ccccttctcg
    13561 ggacctcccg gccactatcc ggatcaattt attcctaact ttgacgcggt aaaggactcg
    13621 gcggatggct acgactgaat gttaagtgga gaggcagagc aactgcgcct gaaacacctg
    13681 gtccactgtc gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa
    13741 ttgcccgagg atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag
    13801 cttgcccgta gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg
    13861 ggaccctgtg ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctt
    13921 tgttgccatc tctgtgctga gtataataaa tacagaaatt aaaatatact ggggctccta
    13981 tcgccatcct gtaaacgcca ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc
    14041 tggtactttt aacatctctc cctctgtgat ttacaacagt ttcaacccag acggagtgag
    14101 tctacgagag aacctctccg agctcagcta ctccatcaga aaaaacacca ccctccttac
    14161 ctgccgggaa cgtacgagtg cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa
    14221 accagacttt ttccggacag acctcaataa ctctgtttac cagaacagga ggtgagctta
    14281 gaaaaccctt agggtattag gccaaaggcg cagctactgt ggggtttatg aacaattcaa
    14341 gcaactctac gggctattct aattcaggtt tctctagaaa tggacggaat tattacagag
    14401 cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa tcaagagctc
    14461 caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc
    14521 aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt gccaaccaag
    14581 cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca gcactcggta
    14641 gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg cacccttatt
    14701 aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa aataataaag
    14761 catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt
    14821 gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct
    14881 aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt
    14941 gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac
    15001 ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt
    15061 tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa
    15121 tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac
    15181 ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct
    15241 ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc
    15301 tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga
    15361 ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct
    15421 gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc
    15481 ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca
    15541 aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac
    15601 tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt
    15661 tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact
    15721 aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca
    15781 aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt
    15841 ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct
    15901 tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa
    15961 tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac
    16021 aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta aactaggaac
    16081 tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct
    16141 aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc
    16201 taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt
    16261 ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat
    16321 aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa
    16381 ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc
    16441 taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca
    16501 agtttactta aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac
    16561 acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc
    16621 tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc
    16681 ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa
    16741 tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac
    16801 cgtaccttaa tcaaactcac agaaccctag tattcaacct gccacctccc tcccaacaca
    16861 cagagtacac agtcctttct ccccggctgg ccttaaaaag catcatatca tgggtaacag
    16921 acatattctt aggtgttata ttccacacgg tttcctgtcg agccaaacgc tcatcagtga
    16981 tattaataaa ctccccgggc agctcactta agttcatgtc gctgtccagc tgctgagcca
    17041 caggctgctg tccaacttgc ggttgcttaa cgggcggcga aggagaagtc cacgcctaca
    17101 tgggggtaga gtcataatcg tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa
    17161 taaactgctg ccgccgccgc tccgtcctgc aggaatacaa catggcagtg gtctcctcag
    17221 cgatgattcg caccgcccgc agcataaggc gccttgtcct ccgggcacag cagcgcaccc
    17281 tgatctcact taaatcagca cagtaactgc agcacagcac cacaatattg ttcaaaatcc
    17341 cacagtgcaa ggcgctgtat ccaaagctca tggcggggac cacagaaccc acgtggccat
    17401 cataccacaa gcgcaggtag attaagtggc gacccctcat aaacacgctg gacataaaca
    17461 ttacctcttt tggcatgttg taattcacca cctcccggta ccatataaac ctctgattaa
    17521 acatggcgcc atccaccacc atcctaaacc agctggccaa aacctgcccg ccggctatac
    17581 actgcaggga accgggactg gaacaatgac agtggagagc ccaggactcg taaccatgga
    17641 tcatcatgct cgtcatgata tcaatgttgg cacaacacag gcacacgtgc atacacttcc
    17701 tcaggattac aagctcctcc cgcgttagaa ccatatccca gggaacaacc cattcctgaa
    17761 tcagcgtaaa tcccacactg cagggaagac ctcgcacgta actcacgttg tgcattgtca
    17821 aagtgttaca ttcgggcagc agcggatgat cctccagtat ggtagcgcgg gtttctgtct
    17881 caaaaggagg tagacgatcc ctactgtacg gagtgcgccg agacaaccga gatcgtgttg
    17941 gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag
    18001 gtgcgggcgt gacaaacaga tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag
    18061 tagttgtagt atatccactc tctcaaagca tccaggcgcc ccctggcttc gggttctatg
    18121 taaactcctt catgcgccgc tgccctgata acatccacca ccgcagaata agccacaccc
    18181 agccaaccta cacattcgtt ctgcgagtca cacacgggag gagcgggaag agctggaaga
    18241 accatgtttt tttttttatt ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa
    18301 gtgaacgcgc tcccctccgg tggcgtggtc aaactctaca gccaaagaac agataatggc
    18361 atttgtaaga tgttgcacaa tggcttccaa aaggcaaacg gccctcacgt ccaagtggac
    18421 gtaaaggcta aacccttcag ggtgaatctc ctctataaac attccagcac cttcaaccat
    18481 gcccaaataa ttctcatctc gccaccttct caatatatct ctaagcaaat cccgaatatt
    18541 aagtccggcc attgtaaaaa tctgctccag agcgccctcc accttcagcc tcaagcagcg
    18601 aatcatgatt gcaaaaattc aggttcctca cagacctgta taagattcaa aagcggaaca
    18661 ttaacaaaaa taccgcgatc ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc
    18721 aggtctgcac ggaccagcgc ggccacttcc ccgccaggaa ccatgacaaa agaacccaca
    18781 ctgattatga cacgcatact cggagctatg ctaaccagcg tagccccgat gtaagcttgt
    18841 tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc
    18901 aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc
    18961 accacagaaa aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca
    19021 aaataaaata acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa
    19081 cccttataag cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac
    19141 cgtgattaaa aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact
    19201 cggtaaacac atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg
    19261 ggggaataca tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa
    19321 aattaatagg agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag
    19381 caccctcccg ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc
    19441 ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc
    19501 agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg
    19561 taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac
    19621 gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca
    19681 cttcccattt taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct
    19741 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
    19801 atattggctt caatccaaaa taaggtatat tattgatgat gttaattaag aattcggatc
    19861 tgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
    19921 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
    19981 tcacggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    20041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    20101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    20161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    20221 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    20281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    20341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    20401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    20461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    20521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    20581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    20641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    20701 atcaaaaagg atcttcacct agatcctttt aaatcaatct aaagtatata tgagtaaact
    20761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    20821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    20881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    20941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    21001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    21061 agtttgcgca acgttgttgc cattgttgca ggcatcgtgg tgtcacgctc gtcgtttggt
    21121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    21181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    21241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    21301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    21361 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
    21421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    21481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    21541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    21601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    21661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    21721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    21781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa