pSMART LCKan质粒

基本信息

原核抗性:	Kan
克隆菌株:	DH5α
培养条件:	LB/37℃

质粒简介

pSMART LCKan质粒

质粒图谱

质粒序列

LOCUS       Exported                1969 bp ds-DNA     circular SYN 20-JUL-2018
DEFINITION  synthetic circular DNA
KEYWORDS    pSMART LCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1969)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      65..96
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        97..187
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             188..1003
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1004..1195
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      1219..1246
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1258..1846)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1868..1897
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc
       61 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      121 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      181 aggagctatg agccatattc aacgggaaac gtcttgctct aggccgcgat taaattccaa
      241 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
      301 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
      361 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
      421 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
      481 cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
      541 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
      601 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
      661 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
      721 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
      781 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
      841 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
      901 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
      961 gaataaattg cagtttcatt tgatgctcga tgagtttttc taaatgacca aacaggaaaa
     1021 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     1081 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     1141 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgatgagg
     1201 gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt
     1261 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1321 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1381 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1441 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1501 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1561 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1621 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1681 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1741 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1801 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1861 tttctaccga agaaaggccc acccgtgaag gtgagccagt gagttgattg cagtccagtt
     1921 acgctggagt ctgaggctcg tcctgaatga tatcaagctt gaattcgtt