- ￥1350 - 3000
- Proteintech
- 10366-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Vimentin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA
- 靶点：
Vimentin
- 抗体英文名：
Vimentin Polyclonal antibody
- 抗体名：
Vimentin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, NIH/3T3 cells, mouse heart tissue, Jurkat cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue, human kidney tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells, mMSCs cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine, hamster, sheep, goat, deer |
| 免疫原 |
CatNo: Ag0489 Product name: Recombinant human Vimentin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC000163 Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vimentin |
| 别名 | VIM |
| 计算分子量 | 466 aa, 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC000163 |
| 基因名称 | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| RRID | AB_2273020 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08670 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Nat Cancer Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinomaAuthors - Xinyao Qiu |
| human | WB | Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.Authors - Jia-Yuan Zhang |
| mouse,human | IF | Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancerAuthors - Yifei Cheng |
| human | WB | ACS Nano Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal TransitionAuthors - Wenjie Wu |
| mouse | IF | Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formationAuthors - Zhenyu Tian |
| human | Adv Sci (Weinh) PMID: 31728273Authors - Qingming Tang |
和程度，并且考虑是否有必要进行半定量测定。 《一》以抗原表达模式定位 1．细胞质内弥散性分布，多数免疫组织化学染色为胞质型阳性反应，如细胞角蛋白(Cytokeratin，CK)和波形蛋白(Vimentin)等。 免疫组织化学染色(SP法。AEC显色) 胚胎12天人员端脑背侧脑室区司见波形蛋L(Vimentin)免疫反应阳性细胞：其胞体位于脑室区。一端伸出长突起到达软脑膜 2．细胞核周边胞质内分布，其判别要点是细胞核轮廓被勾画得很清楚，如CD3多克隆抗体的染色
，多数胞浆型抗体的反应如此，如细胞角蛋白（cytokeratin，CK）和波形蛋白（vimentin）等； （2）细胞核周的胞浆内分布，其判别要点是细胞核的轮廓被勾画得很清楚，如CD3多克隆抗体的染色； （3）胞浆内局限性点状阳性，如CDl5抗体的染色； （4）细胞膜线性阳性，大多数淋巴细胞标记的染色如此，如CD20、CD45RO； （5）细胞核阳性，如Ki-67及雌、孕激素受体蛋白ER、PR等。一种抗体可同时出现细胞浆和细胞膜的阳性表达，如EMA可呈膜性和胞浆内弥漫性阳性反应；CD30抗体
角蛋白(cytokeratin，CK)和波形蛋白(vimentin)等；2）细胞核周的胞浆内分布，其判别要点是细胞核的轮廓被勾画得很清楚，如CD3多克隆抗体的染色；3）胞浆内局限性点状阳性，如CDl5抗体的染色；4）细胞膜线性阳性，大多数淋巴细胞标记的染色如此，如CD20、CD45RO；5）细胞核阳性，如Ki-67及雌、孕激素受体蛋白ER、PR等。一种抗体可同时出现细胞浆和细胞膜的阳性表达，如EMA可呈膜性和胞浆内弥漫性阳性反应；CD30抗体可同时呈膜性和胞浆内点状阳性反应等。（7） 对照
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