手机验证
云舟生物科技(广州)股份有限公司
定制载体、对照载体
血清型 | 组织嗜性 |
---|---|
AAV1 | 平滑肌、骨骼肌、中枢神经系统、脑、肺、视网膜、内耳、胰腺、心脏、 肝脏 |
AAV2 | 平滑肌、中枢神经系统、大脑、肝脏、胰腺、肾脏、视网膜、内耳、睾丸 |
AAV3 | 平滑肌、肝脏、 肺 |
AAV4 | 中枢神经系统、 视网膜、肺、肾、心脏 |
AAV5 | 平滑肌、中枢神经系统、脑、肺、视网膜、心脏 |
AAV6 | 平滑肌、心脏、 肺、胰腺、脂肪、肝脏 |
AAV6.2 | 肺、肝、内耳 |
AAV7 | 平滑肌、视网膜、中枢神经系统、大脑、肝脏 |
AAV8 | 平滑肌、中枢神经系统、脑、视网膜、内耳、肝脏、胰腺、心脏、肾脏、脂肪 |
AAV9 | 平滑肌、骨骼肌、肺、肝、心脏、胰腺、 中枢神经系统、视网膜、内耳、睾丸、肾脏、脂肪 |
AAVrh10 | 平滑肌、肺、肝、心脏、胰腺、中枢神经系统、视网膜、肾脏 |
AAV-DJ | 肝、心、肾、脾 |
AAV-DJ/8 | 肝、脑、脾、肾 |
AAV-P-HPs.eB | 中枢神经系统 |
AAV-P-HPs。S | PNS |
AAV2-复古 | 脊神经 |
AAV2-四头肌 | 内皮细胞,视网膜 |
AAV2.7m8 | 视网膜,内耳 |
难以转导某些细胞类型: 我们的AAV载体系统可以转导许多不同的细胞类型,包括包装成适当血清型的非分裂细胞。然而,不同的AAV血清型对不同的细胞类型具有嗜性,并且某些细胞类型可能难以被任何血清型转导。
技术复杂性:使用病毒载体需要在包装细胞中产生活病毒,然后测量病毒滴度。与传统质粒转染相比,这些程序在技术上要求很高且耗时。这些需求可以通过在订购载体时选择我们的病毒包装服务来缓解。
PAM 要求: 我们的AAV CRISPR载体旨在与源自金黄色-葡萄球菌的SaCas9配合使用。SaCas9介导的CRISPR靶向取决于PAM序列的存在,NNGRR(首-选NNGRRT)位于gRNA识别序列的紧邻3'端。
5' ITR: 5' inverted terminal repeat. In wild type virus, 5' ITR and 3' ITR are essentially identical in sequence. They reside on two ends of the viral genome pointing in opposite directions, where they serve as the origin of viral genome replication.
U6 Promoter: Drives expression of the downstream SagRNA sequence. This is the promoter of the human U6 snRNA gene, an RNA polymerase III promoter which efficiently expresses short RNAs.
Guide sequence: Specifies the target sequence for the SaCas9 nuclease.
gRNA scaffold: Structural portion of the gRNA to allow complexing with SaCas9.
Terminator: Terminates transcription of the gRNA.
CMV promoter: Human cytomegalovirus immediate early enhancer/promoter. It drives the ubiquitous expression of the downstream SaCas9 gene.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes.
SaCas9: SaCas9 nuclease derived from Staphylococcus aureus.
BGH pA: Bovine growth hormone polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
3' ITR: 3' inverted terminal repeat. See description for 5’ ITR.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
引用 | 主题 |
---|---|
科学。339:819 (2013) | 使用CRISPR/Cas9系统进行基因组编辑的描述 |
Genome Biol. 16:257 (2015) | Characterization of Staphylococcus aureus Cas9 |
Nature. 520:186 (2015) | In vivo genome editing with SaCas9-based AAV vectors |