手机验证
云舟生物科技(广州)股份有限公司
病毒包装、辅助病毒、对照病毒
>1 x 10^10 IFU/ml,10 x 25 ul
我们的滴度保证仅适用于包装进病毒的区域(从5’ ITR 到3’ ITR)小于腺病毒承载限度(38.7 kb,~野生型HAd5基因组长度36 kb的108%)的载体。超过该范围,超出的那部分序列在包入衣壳时会被剪切掉,导致包装出来的腺病毒颗粒含有不完整的genome。此外,对于如下载体,我们同样不能保证滴度:
We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.
Alexander Mayer
University of Würzburg, Germany
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