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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human RTN4 / NOGO-A Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50.00 µg/100.00 µg
| 规格: | 50.00 µg | 产品价格: | ¥2310.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:RTN4蛋白, RTN4 protein
蛋白构建:A DNA sequence encoding the human RTN4 (NP_065393.1) N-terminal fragment (Met 1-Val 185) was fused with the GST tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 70 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human RTN4/GST chimera consists of 417 amino acids and has a predicted molecular mass of 46.2 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_065393.1
蛋白氨基酸序列:Met1-Val185
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Liu, Y; et al. Nogo-A exacerbates sepsis-associated encephalopathy by modulating microglial SHP-2/NLRP3 balance and inducing ROS and M1 polarization. Biomolecules & biomedicine, PMID: 39151100
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
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