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50 µg
蛋白名称:Doublecortin/DCX蛋白, Doublecortin/DCX protein
蛋白构建:A DNA sequence encoding the human DCX (O43602-2) N-terminal fragment (Ala 45-Val 150) was fused with the GST tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 82 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human DCX (aa 45-150)/GST chimera consists of 340 amino acids and has a predicted molecular mass of 39.4 kDa. It migrates as an approxiamtely 36 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:O43602-2
蛋白氨基酸序列:Ala45-Val150
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Cell Lineage Analysis: X-Inactivation Mosaics
members of this line express the X-linked lacZ gene in all cells of the embryo, including the developing nervous system (5 ). Ubiquitous expression of the lacZ gene produces a nuclear-localized β-gal protein, which, after histochemical reaction with X-gal
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Immunocytochemical Analysis of Neuronal Differentiation
Doublecortin (Dcx), the early expressed marker Hu, and mature neuronal marker NeuN. In Chapters 25 and 27 of Part IV, we provide protocols for identifying newborn cells by using the mitotic label bromodeoxyuridine and for examining axonal projections by using
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