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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse MIF / Migration Inhibitory Factor Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥1080.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥1780.0 |
蛋白名称:MIF蛋白, MIF protein
蛋白构建:A DNA sequence encoding the mouse MIF (P34884) precursor (Pro 2-Ala 115) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Pro 2
蛋白分子量:The secreted recombinant mouse MIF consists of 125 amino acids and has a calculated molecular mass of 13.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 18 and 22 kDa in SDS-PAGE under reducing conditions due to different glycosylation.
蛋白NP号:P34884
蛋白氨基酸序列:Pro2-Ala115
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验mES cell growth medium mES cell growth medium with Leukemia Inhibitory Factor (LIF) OPTI-MEM (+HEPES, +sodium bicarbonate, +glutamine) (GIBCO/Invitrogen) Sleeping Beauty transposon and transposase plasmids (available from the Center
Culture of Mouse Olfactory Sensory Neurons
. Cell. Dev. Biol. 22:713‐737. Moon, C., Yoo, J.Y., Matarazzo, V., Sung, Y.K., Kim, E.J., and Ronnett, G.V. 2002. Leukemia inhibitory factor inhibits neuronal terminal
Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. 1990. Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev. Biol. 141:344‐352.
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