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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Vitronectin / VTN Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥10800.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥380.0 |
| 规格: | 100.00 µg | 产品价格: | ¥1380.0 |
| 规格: | 500.00 µg | 产品价格: | ¥1380.0 |
蛋白名称:Vitronectin蛋白, Vitronectin protein
蛋白构建:A DNA sequence encoding the mouse vitronectin (NP_035837.1) (Met 1-Lys 478) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37 ℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp 20
蛋白分子量:The secreted recombinant mouse vitronectin consists of 470 amino acids and has a predicted molecular mass of 54.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmvitronectin is approximately 75-85 kDa due to glycosylation.
蛋白NP号:NP_035837.1
蛋白氨基酸序列:Met1-Lys478
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐affinity tag antibodies for development and determination of protein display, 2624B (Cell Signaling Technology) Hybridization chamber (Corning, product no. 2551
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
detailed insight in considerations to choose and set up the appropriate mouse model for specific vascular research questions. Additionally, we provide technical details to execute experiments with these animal models. Curr. Protoc. Mouse Biol. 2:317?345 ©
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