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- 规格:
100 µg
蛋白名称:ICAM-2蛋白, ICAM-2 protein
蛋白构建:A DNA sequence encoding the mouse ICAM2 (NP_034624.1) extracellular domain (Met 1-Gln 222) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 93 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells . When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 20
蛋白分子量:The secreted recombinant mouse ICAM2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, the apparent molecular mass of rm ICAM2/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_034624.1
蛋白氨基酸序列:Met1-Gln222
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Purpose The purification of ICAM-1 on R6.5 anti-ICAM-1 column. Materials Triethylamine NaCl Octyl Glucoside ( n-octyl-b -D-glucopyranoside Sigma Cat. No. O-8001) Tris-Base NaN3 Tris-HCl
ICAM-1纯化 Purification of ICAM-1
Purification of ICAM-1 by Mazen Ferzly, 06/22/2000 Purpose The purification of ICAM-1 on R6.5 anti-ICAM-1 column. Materials Triethylamine NaCl Octyl Glucoside ( n-octyl-b -D-glucopyranoside Sigma Cat
ELISA FOR DETECTING SECRETED ICAM-1
substrate and substrate buffer (Zymed; #00-2011) ABTS chromogen solution Hydrogen Peroxidase solution Citrate buffer Anti-ICAM-1 mAb R6.5 or CL203. Use as 10 ug/ml in PBS to coat plates. Biotinylated mAb
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