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100 µg
蛋白名称:ICAM-2蛋白, ICAM-2 protein
蛋白构建:A DNA sequence encoding the mouse ICAM2 (NP_034624.1) (Met 1-Gln 222)was expressed with six amino acids (LEVLFQ) at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to rmICAM1 coated plates (12.5 μg/mL with 100 μL/well), > 30% cells will adhere after 1 hour incubation at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 20
蛋白分子量:The recombinant mouse ICAM2 consists of 210 amino acids and has a calculated molecular mass of 23.6 kDa. The recombinant protein migrates as an approximately 38-42 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_034624.1
蛋白氨基酸序列:Met 1-Gln 222
蛋白标签:C-cleavage
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Purpose The purification of ICAM-1 on R6.5 anti-ICAM-1 column. Materials Triethylamine NaCl Octyl Glucoside ( n-octyl-b -D-glucopyranoside Sigma Cat. No. O-8001) Tris-Base NaN3 Tris-HCl
ICAM-1纯化 Purification of ICAM-1
Purification of ICAM-1 by Mazen Ferzly, 06/22/2000 Purpose The purification of ICAM-1 on R6.5 anti-ICAM-1 column. Materials Triethylamine NaCl Octyl Glucoside ( n-octyl-b -D-glucopyranoside Sigma Cat
中,现在Invitrogen已经克服了这个问题。在载体上多加四个碱基GTGG,就是这四个碱基使它可以与PCR产物定向连接。其作用机理见下图。 结合TOPO技术的有五个系列载体:pET100/D-TOPO®、pET101/D-TOPO®、pET102/D-TOPO®、pET151/D-TOPO®和pET200/D-TOPO®,这五个载体根据所带的标记、是否具有蛋白酶切位点而各有不同。详细信息参见下图。 结合TOPO技术的有五个系列载体:pET100/D
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