重组人 R-Spondin 1 / RSPO1 蛋白 (aa 1-146, His标签)

重组人 R-Spondin 1 / RSPO1 蛋白 (aa 1-146, His标签)（产品说明）
蛋白名称：Human R-Spondin 1 / RSPO1 Protein (aa 1-146, His Tag)
蛋白构建：A DNA sequence encoding the amino acids (Met 1-Ala 146) of human RSPO1 (NP_001033722.1) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. 2. Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 21
蛋白分子量：The secreted recombinant human RSPO1 (aa 1-146) comprises 137 amino acids and has a predicted molecular mass of 14 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001033722.1
蛋白氨基酸序列：Met1-Ala146
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.