- ￥580 - 29180
- Sino Biological
- 北京
- 10704-HNAS
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Interferon beta / IFN-beta / IFNB Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥29180.0 |
|---|---|---|---|
| 规格： | 5.00 µg | 产品价格： | ￥580.0 |
| 规格： | 20.00 µg | 产品价格： | ￥1980.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4480.0 |
| 规格： | 500.00 µg | 产品价格： | ￥18480.0 |
蛋白名称：Human Interferon beta / IFN-beta / IFNB Protein
蛋白构建：A DNA sequence encoding the human IFNβ (P01574) (Met1-Asn187) was expressed.
表达宿主：CHO Stable Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 2-20 pg/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met 22
蛋白分子量：The recombinant human IFNβ consists of 166 amino acids and predicts a molecular mass of 20 KDa. It migrates as an approximately 20-23 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P01574
蛋白氨基酸序列：Met1-Asn187
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Qiu, Y; et al. Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals. Immunity, PMID: 28636969
3, Dai, T; et al. FAF1 Regulates Antiviral Immunity by Inhibiting MAVS but Is Antagonized by Phosphorylation upon Viral Infection. Cell host & microbe, PMID: 30472208
4, Yang, C; et al. LYAR suppresses interferon-β induction by targeting phosphorylated IRF3. Journal of virology, PMID: 31413131
5, Zhang, Z; et al. Acetylation-Dependent Deubiquitinase OTUD3 Controls MAVS Activation in Innate Antiviral Immunity. Molecular cell, PMID: 32679077
6, Fu, Z; et al. The complex structure of GRL0617 and SARS-CoV-2 PLpro reveals a hot spot for antiviral drug discovery. Nature communications, PMID: 33473130
7, Chiok, K; et al. Human Respiratory Syncytial Virus NS2 Protein Induces Autophagy by Modulating Beclin1 Protein Stabilization and ISGylation. mBio, PMID: 35038909
8, Zhang, Y; et al. Allelic imbalance of HLA-B expression in human lung cells infected with coronavirus and other respiratory viruses. European journal of human genetics : EJHG, PMID: 35322240
9, Cao, D; et al. The SARS-CoV-2 papain-like protease suppresses type I interferon responses by deubiquitinating STING. Science signaling, PMID: 37130168
10, Zhang, Y; et al. Enterovirus D68 infection upregulates SOCS3 expression to inhibit JAK-STAT3 signaling and antagonize the innate interferon response of the host. Virologica Sinica, PMID: 37657555
11, Zou, J; et al. Prohibitin1 facilitates viral replication by impairing the RIG-I-like receptor signaling pathway. Journal of virology, PMID: 37754758 1
2, Yu, S-S; et al. Deacetylase Sirtuin 1 mitigates type I IFN- and type II IFN-induced signaling and antiviral immunity. Journal of virology, PMID: 38386781 1
3, Zhou, M; et al. Optimizing GBM organoid construction with hydrogel-based models: GelMA-HAMA scaffold supports GBM organoids with clonal growth for drug screening. Cell transplantation, PMID: 40556129
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment
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