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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Interferon beta / IFN-beta / IFNB Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥29180.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1980.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4480.0 |
| 规格: | 500.00 µg | 产品价格: | ¥18480.0 |
蛋白名称:Human Interferon beta / IFN-beta / IFNB Protein
蛋白构建:A DNA sequence encoding the human IFNβ (P01574) (Met1-Asn187) was expressed.
表达宿主:CHO Stable Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 2-20 pg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met 22
蛋白分子量:The recombinant human IFNβ consists of 166 amino acids and predicts a molecular mass of 20 KDa. It migrates as an approximately 20-23 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P01574
蛋白氨基酸序列:Met1-Asn187
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment
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