CD32A/FCGR2A重组蛋白|Recombinant Human CD32a/FCGR2A Protein(167 His,His&AVI Tag),Biotinylated
- ￥2250 - 5640
- Sino Biological
- 北京
- 10374-H27H1-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD32a/FCGR2A Protein(167 His,His&AVI Tag),Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg/100.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥2250.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥4520.0 |
| 规格： | 100.00 µg | 产品价格： | ￥5640.0 |
蛋白名称：CD32A蛋白, CD32A protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR2A131H/R in the reference.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated(Cat. 10374-H27H1-B) bound to Avastin was 0.03 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 34
蛋白分子量：The recombinant human CD32aconsists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
蛋白NP号：P12318-1
蛋白氨基酸序列：Met1-Met210
蛋白标签：C-AVI & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.Molecular immunology, PubMed ID: 32947169
3, Riechert V, et al. FcγRIIA-specific DARPins as novel tools in blood cell analysis and platelet aggregation.The Journal of biological chemistry, PubMed ID: 37100283
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Protein Components of the microRNA Pathway and Human Diseases
, Exportin-5 (Exp-5), TAR RNA binding protein (TRBP) and fragile X mental retardation protein (FMRP), are each related, in various ways, to specific genetic diseases.
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
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