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- SA-02-BT
- 美国
- 2026年01月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Minute™ Total Protein Extraction Kit for Bone Tissue
- 保存条件：
室温
- 规格：
50 Preps
MinuteTM 骨组织总蛋白提取试剂盒
目录号：SA-02-BT
描述： 骨组织通常用于研究的有两种类型：密质骨和松质骨。骨组织中的细胞紧密堆积，由于其独特的结构，很难有效地从中提取总蛋白质。使用传统的溶液法提取蛋白质如RIPA效率低，产率也很低，提取的蛋白质图谱也是不完整的。本试剂盒利用离心管技术以及优化的裂解液，利用物理研磨和化学裂解相结合，提供了一种简单和快速的从人或动物骨组织中提取蛋白质的高效方法。可根据特定的下游应用选择变性裂解液或天然裂解液。整个过程不到10分钟即可完成，蛋白质得率根据骨骼样品类型不同在0.5-2mg/ml范围。试剂盒所提供试剂及耗材可以满足50个样品实验。
应用：
SDS-PAGE, WB，IP，ELISA，酶活性测定和蛋白质组学分析。缓冲剂与IMAC树脂兼容，可用于His标签蛋白纯化。在质谱分析之前需将提取的蛋白质样品中的盐和表面活性剂除去。
参考文献：
1. Ren, H., Wang, Z., Xu, J., Chen, J., & Lan, J. (2020). The Impact of Frizzled-9 on Dental Implant Osseointegration in Hyperlipidemic Rats. Journal of Hard Tissue Biology, 29(1), 37-44.
2. Pedersen, K. B., Osborn, M. L., Robertson, A. C., Williams, A. E., Watt, J., Denys, A., ... & Ronis, M. J. (2020). Chronic Ethanol Feeding in Mice Decreases Expression of Genes for Major Structural Bone Proteins in a Nox4-Independent Manner. Journal of Pharmacology and Experimental Therapeutics, 373(3), 337-346.
3.He-dong Liu, Mao-xian Ren, Yang Li, Ruo-tian Zhang, Neng-feng Ma, Tianlin Li, Wen-kai Jiang, Zhi Zhou, Xue-wei Yao, Zhi-yi Liu & Min Yang (2022): Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells and promotes osteogenesis by activating SIRT1, Free Radical Research, DOI: 10.1080/10715762.2022.2037580
4.Chaolai Jiang; Yiwei Lin; Haojie Shan; Wenyang Xia; Chenhao Pan; et al(2022).miR-146a Protects against Staphylococcus aur eus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis.ACS Infectious Diseases.
5.Song, D., Ha, C. Z., Xu, Q., & Hu, Y. H. (2022). mRNA expression profiling of the cancellous bone in patients with idiopathic osteonecrosis of the femoral head by whole-transcriptome sequencing. Medicine, 101(35), e30213.
6.Deng, J., Cohen, D. J., Sabalewski, E. L., Van Duyn, C., Wilson, D. S., Schwartz, Z., & Boyan, B. D. (2022). Semaphorin 3A Delivered by a Rapidly Polymerizing Click Hydrogel Overcomes Impaired Implant Osseointegration in a Rat Type 2 Diabetes Model. Acta Biomaterialia.
7.He, H., Zhang, Y., Sun, Y., Zhang, Y., Xu, J., Yang, Y., & Chen, J. (2022). Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway. Frontiers in cell and developmental biology, 9, 3661.
8.Choi, R. Y., Kim, I. W., Ji, M., Paik, M. J., Ban, E. J., Lee, J. H., ... & Seo, M. (2023). Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice. Biomedicine & Pharmacotherapy, 165, 115112.
9.Liu, Z., Yao, X., Jiang, W., Zhou, Z., & Yang, M. (2023). Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα/NOX4/ROS/NF-κB pathways. Journal of Orthopaedic Surgery and Research, 18(1), 1-14.
10.Zhang, C. X., & Man, Q. W. (2024). Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling. British Journal of Oral and Maxillofacial Surgery.
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- 作者
- 内容
- 询问日期
文献和实验1. Ren, H., Wang, Z., Xu, J., Chen, J., & Lan, J. (2020). The Impact of Frizzled-9 on Dental Implant Osseointegration in Hyperlipidemic Rats. Journal of Hard Tissue Biology, 29(1), 37-44.
2. Pedersen, K. B., Osborn, M. L., Robertson, A. C., Williams, A. E., Watt, J., Denys, A., ... & Ronis, M. J. (2020). Chronic Ethanol Feeding in Mice Decreases Expression of Genes for Major Structural Bone Proteins in a Nox4-Independent Manner. Journal of Pharmacology and Experimental Therapeutics, 373(3), 337-346.
3.He-dong Liu, Mao-xian Ren, Yang Li, Ruo-tian Zhang, Neng-feng Ma, Tianlin Li, Wen-kai Jiang, Zhi Zhou, Xue-wei Yao, Zhi-yi Liu & Min Yang (2022): Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells and promotes osteogenesis by activating SIRT1, Free Radical Research, DOI: 10.1080/10715762.2022.2037580
4.Chaolai Jiang; Yiwei Lin; Haojie Shan; Wenyang Xia; Chenhao Pan; et al(2022).miR-146a Protects against Staphylococcus aur eus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis.ACS Infectious Diseases.
5.Song, D., Ha, C. Z., Xu, Q., & Hu, Y. H. (2022). mRNA expression profiling of the cancellous bone in patients with idiopathic osteonecrosis of the femoral head by whole-transcriptome sequencing. Medicine, 101(35), e30213.
6.Deng, J., Cohen, D. J., Sabalewski, E. L., Van Duyn, C., Wilson, D. S., Schwartz, Z., & Boyan, B. D. (2022). Semaphorin 3A Delivered by a Rapidly Polymerizing Click Hydrogel Overcomes Impaired Implant Osseointegration in a Rat Type 2 Diabetes Model. Acta Biomaterialia.
7.He, H., Zhang, Y., Sun, Y., Zhang, Y., Xu, J., Yang, Y., & Chen, J. (2022). Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling Pathway. Frontiers in cell and developmental biology, 9, 3661.
8.Choi, R. Y., Kim, I. W., Ji, M., Paik, M. J., Ban, E. J., Lee, J. H., ... & Seo, M. (2023). Protaetia brevitarsis seulensis larvae ethanol extract inhibits RANKL-stimulated osteoclastogenesis and ameliorates bone loss in ovariectomized mice. Biomedicine & Pharmacotherapy, 165, 115112.
9.Liu, Z., Yao, X., Jiang, W., Zhou, Z., & Yang, M. (2023). Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα/NOX4/ROS/NF-κB pathways. Journal of Orthopaedic Surgery and Research, 18(1), 1-14.
10.Zhang, C. X., & Man, Q. W. (2024). Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling. British Journal of Oral and Maxillofacial Surgery.
11.Zhang, C. X., & Man, Q. W. (2024). Proteomics study of bone tissue around ameloblastoma and the potential mechanism of CD36 in bone remodelling. British Journal of Oral and Maxillofacial Surgery.
干燥。以上三种方法的共同问题是操作繁琐，产量低。 C. 离心管柱提取法： 这种方法利用了一种新的蛋白质提取技术，脂肪组织通过无表面活性剂的缓冲液匀浆，水油乳状液通过简单的冷冻后，离心使其通过特殊的离心管柱（有孔径，表面性质被处理过的）提取。离心管柱的作用是快速，干净的从油相中分离出水相蛋白。Invent Biotechnologies Inc 公司利用专利技术生产的这种脂肪蛋白提取试剂盒可以从脂肪组织中提取全部的总蛋白，产量远远高于其他方法。 推荐产品：Minute™ Total
Matrix 310 Matrix 3100 NGF Emax免疫测试系统 pGEM-T和pGEM-T Easy 载体系统 Powerplex 16 组织和毛发提取试剂盒 siLentGene™ U6 盒 RNA 干扰系统
）从倍增基因获得，也可以来自人工合成的基因。通过PCR获得感兴趣的DNA的方法是：首先，分离含有感兴趣的DNA片段的mRNA的总RNA，并通过逆转录聚合酶链反应（RT-PCR）将RNA逆转录成cDNA。根据公布的NCBI基因编码序列（CD）设计寡核苷酸引物，并在引物中加入指定的限制酶位点。然后，通过PCR扩增感兴趣的DNA的编码区，用凝胶提取试剂盒纯化后，将DNA片段插入克隆载体中。通过RT-PCR将RNA逆转录成cDNA通过PCR扩增感兴趣的DNA的编码区经验虽然从RT-PCR中获取DNA片段是常用
