- ￥4570
- Invent
- SA-01-SK
- 美国
- 2026年01月07日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Minute™ Total Protein Extraction Kit for Skin Tissue
- 保存条件：
室温
- 规格：
50 Preps
MinuteTM 皮肤组织总蛋白提取试剂盒
目录号：SA-01-SK
描述
皮肤组织由表皮、真皮和皮下脂肪组成。由于皮肤组织独特的结构，所以研磨匀浆非常困难，在提取总蛋白时也十分难裂解。使用传统的溶液蛋白质提取方法如RIPA，提取皮肤总蛋白效率，产率都极低。提取的蛋白质图谱也是不完整的。本试剂盒使用离心管柱技术及优化的裂解液，采取物理研磨和化学裂解相结合的方法，为人和动物皮肤组织总蛋白的提取提供了一种高效的方法。本试剂盒可简单快速的提取高质量皮肤总蛋白，无蛋白丢失，可以根据特定的下游应用选择变性细胞裂解液或天然细胞裂解液。整个提取过程不到10分钟即可完成，蛋白质得率在1-5mg/ml。试剂盒所提供试剂及耗材可以满足客户50个样品实验。
应用
SDS-PAGE, WB，IP，ELISA，酶活性测定和蛋白质组学分析。缓冲剂与IMAC树脂兼容，可用于His标签蛋白纯化。在质谱分析之前需将提取的蛋白质样品中的盐和表面活性剂除去。
参考文献：
1.Zhang, C., Li, Y., Qin, J., Yu, C., Ma, G., Chen, H., & Xu, X. (2021). TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration. Frontiers in Pharmacology, 12.
2.Wang, T., Zhu, G., Qin, L., Wang, Q., She, C., Xu, D., ... & Li, S. T. (2021). Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innovations, 1(3), 100038.
3.Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., ... & Wang, Y. (2022). Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Communication and Signaling, 20(1), 1-18.
4.Wang, Y., Huang, J., & Jin, H. (2022). Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model. International Journal of Molecular Sciences, 23(20), 12672.
5.Zhu, W., Dong, Y., Xu, P., Pan, Q., Jia, K., Jin, P., ... & Che, B. (2022). A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice. Acta Biomaterialia.
6.Yuan, X., Tang, B., Chen, Y., Zhou, L., Deng, J., Han, L., ... & Wang, Y. (2023). Celastrol inhibits store operated calcium entry and suppresses psoriasis. Frontiers in Pharmacology, 14.
7.Zhou, H., Gan, Y., Li, Y., Chen, X., Guo, Y., & Wang, R. (2023). Degradation of Rat Sarcoma Proteins Targeting the Post-Translational Prenyl Modifications via Cascade Azidation/Fluorination and Click Reaction. Journal of Medicinal Chemistry.
8.Wang, J. Study on the Regulatory Mechanism of Jia Wei Guo Min Decoction On Degranulation of Mast Cell in Ad Mice Based on His/Par-2 Pathway.
9.Huang, J. L., Xu, Y. H., Yang, X. W., Wang, J., Zhu, Y., & Wu, X. B. (2023). Jiawei guomin decoction regulates the degranulation of mast cells in atopic dermatitis mice via the HIS/PAR-2 pathway. Journal of Ethnopharmacology, 117485.
10.Cui J, Zhao Q, Song Z, Chen Z, Zeng X, Wang C, Lin Z, Wang F, Yang Y. KLHL24-Mediated Hair Follicle Stem Cells Structural Disruption Causes Alopecia. J Invest Dermatol. 2022 Aug;142(8):2079-2087.e8. doi: 10.1016/j.jid.2022.01.007. Epub 2022 Jan 21. PMID: 35066002.
11.Wu, Z., Liu, C., Yin, S., Ma, J., Sun, R., Cao, G., ... & Wang, Y. (2024). P75NTR regulates autophagy through the YAP-mTOR pathway to increase the proliferation of interfollicular epidermal cells and promote wound healing in diabetic mice. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 167012.
12.Xu, X. L., Wu, S. J., Qi, S. Y., Chen, M. M., Liu, Z. M., Zhang, R., ... & Lian, Z. X. (2024). Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep. Cells, 13(11), 985.
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- 作者
- 内容
- 询问日期
文献和实验1.Zhang, C., Li, Y., Qin, J., Yu, C., Ma, G., Chen, H., & Xu, X. (2021). TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration. Frontiers in Pharmacology, 12.
2.Wang, T., Zhu, G., Qin, L., Wang, Q., She, C., Xu, D., ... & Li, S. T. (2021). Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innovations, 1(3), 100038.
3.Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., ... & Wang, Y. (2022). Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Communication and Signaling, 20(1), 1-18.
4.Wang, Y., Huang, J., & Jin, H. (2022). Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model. International Journal of Molecular Sciences, 23(20), 12672.
5.Zhu, W., Dong, Y., Xu, P., Pan, Q., Jia, K., Jin, P., ... & Che, B. (2022). A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice. Acta Biomaterialia.
6.Yuan, X., Tang, B., Chen, Y., Zhou, L., Deng, J., Han, L., ... & Wang, Y. (2023). Celastrol inhibits store operated calcium entry and suppresses psoriasis. Frontiers in Pharmacology, 14.
7.Zhou, H., Gan, Y., Li, Y., Chen, X., Guo, Y., & Wang, R. (2023). Degradation of Rat Sarcoma Proteins Targeting the Post-Translational Prenyl Modifications via Cascade Azidation/Fluorination and Click Reaction. Journal of Medicinal Chemistry.
8.Wang, J. Study on the Regulatory Mechanism of Jia Wei Guo Min Decoction On Degranulation of Mast Cell in Ad Mice Based on His/Par-2 Pathway.
9.Huang, J. L., Xu, Y. H., Yang, X. W., Wang, J., Zhu, Y., & Wu, X. B. (2023). Jiawei guomin decoction regulates the degranulation of mast cells in atopic dermatitis mice via the HIS/PAR-2 pathway. Journal of Ethnopharmacology, 117485.
10.Cui J, Zhao Q, Song Z, Chen Z, Zeng X, Wang C, Lin Z, Wang F, Yang Y. KLHL24-Mediated Hair Follicle Stem Cells Structural Disruption Causes Alopecia. J Invest Dermatol. 2022 Aug;142(8):2079-2087.e8. doi: 10.1016/j.jid.2022.01.007. Epub 2022 Jan 21. PMID: 35066002.
11.Wu, Z., Liu, C., Yin, S., Ma, J., Sun, R., Cao, G., ... & Wang, Y. (2024). P75NTR regulates autophagy through the YAP-mTOR pathway to increase the proliferation of interfollicular epidermal cells and promote wound healing in diabetic mice. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 167012.
12.Xu, X. L., Wu, S. J., Qi, S. Y., Chen, M. M., Liu, Z. M., Zhang, R., ... & Lian, Z. X. (2024). Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep. Cells, 13(11), 985.
13.Haiyang Hu; Qianglong Sheng; Fan Yang; Xinyi Wu; Youlai Zhang; et al.(2025). Enhanced Skin Wound Healing Through Chemically Modified Messenger RNA Encoding Epidermal Growth Factor (EGF). International Wound Journal.DOI 10.1111/iwj.70143
14.Jiyeon Lee; Jun Kim; Jisun Kim; Soo‐Chang Song.(2025).Synergistic Effect of Prolonged Oxygenation and Reactive Oxygen Species Scavenging on Diabetic Wound Healing Using an Injectable Thermoresponsive Hydrogel.Small.DOI 10.1002/smll.202502399
15. Sugandhika G Welikadage; Habtamu B. Derseh; Trent Perry; Clare A. Anstead; Jean‐Pierre Y. Scheerlinck; et al.(2025).Characterising the innate immune response in breech strike resistant and non-selected sheep to the sheep blowfly (Lucilia cuprina).Veterinary Immunology and Immunopathology.DOI 10.1016/j.vetimm.2025.110982
16.Xingyu Zhang; Jize Dong.(2025). Accelerating Early-Stage Skin Wound Healing with Atomically Hydrogenated Silicene Nanosheets. ACS applied nano materials.DOI 10.1021/acsanm.5c02531
Matrix 310 Matrix 3100 NGF Emax免疫测试系统 pGEM-T和pGEM-T Easy 载体系统 Powerplex 16 组织和毛发提取试剂盒 siLentGene™ U6 盒 RNA 干扰系统
）从倍增基因获得，也可以来自人工合成的基因。通过PCR获得感兴趣的DNA的方法是：首先，分离含有感兴趣的DNA片段的mRNA的总RNA，并通过逆转录聚合酶链反应（RT-PCR）将RNA逆转录成cDNA。根据公布的NCBI基因编码序列（CD）设计寡核苷酸引物，并在引物中加入指定的限制酶位点。然后，通过PCR扩增感兴趣的DNA的编码区，用凝胶提取试剂盒纯化后，将DNA片段插入克隆载体中。通过RT-PCR将RNA逆转录成cDNA通过PCR扩增感兴趣的DNA的编码区经验虽然从RT-PCR中获取DNA片段是常用
电泳时加样量不一样对结果有影响吗 1ml抗凝血要用多少红细胞裂解液？ RETN基因的PCR-RFLP 用CpGenome™ DNA Modification Kit 做BSP QIAGEN试剂盒 Flexigene DNA system的原理 部分甲基化和超甲基化是什么意思 PCR扩增转座子引物设计策略 pcr-sscp如何变性? 单个细胞 21非整倍体分析 线性质粒与环状质粒的扩增效率会不同吗 请帮忙看看这一步是什么意义 RT-PCR半定量结果需要重复吗，如何重复及分析
