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ED-028 Minute™ 内体和细胞组分分离试剂盒

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  • ¥7300
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  • 美国
  • ED-028
  • 2026年04月23日
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      4度

    • 英文名

      Minute™ Endosome Isolation and Cell Fractionation Kit

    • 规格

      20 Preps

    MinuteTM  内体和细胞组分分离试剂盒

     

    目录号:ED-028

    描述:

            初级内体(EE)是次级成熟内体的起点,初级内体主要是由内吞的囊泡相互融合形成。初级内体不仅仅通过网格蛋白介导的信号通路接受胞吞物,还有其他许多通路。胞吞机制除了在维持正常细胞生理中起到重要作用,还在阿尔兹海默病和遗传性溶酶体储积症等许多疾病中发挥了很大作用。传统分离内体的方法是采用密度梯度超速离心法,需要大量起始原材料,方法繁琐费时。Minute™内体分离试剂盒基于离心管柱法,快速简单,仅需要少量的培养细胞或者毫克级的组织样品。  

     

    应用:

    本试剂盒可以从培养的细胞和组织样品中大量沉淀富集初级内体,用于SDS-PAGE、immunoblottings、ELISA、IP、MS、2-D、酶活性检测等其他应用。

     

    产品细节图片1

    试剂盒组分(20 preps):
    1. 缓冲液 A 15ml
    2. 缓冲液 B 15ml
    3.塑料研磨棒 2 根
    4.离心管柱 20 个
    5.收集管 20 个
    6. 组织分离粉 2.5 克

    重要产品信息:
    1. 使用前仔细阅读整个操作说明,并将离心管柱和接收管套管放置于冰上预冷。
    2. 所有离心步骤都需要在 4℃室温下或者低温离心机中进行。
    3. 研究蛋白磷酸化,磷酸酶抑制剂应在使用前加入缓冲液 A 中。蛋白酶抑制剂可以选择添加或不添加,如添加在使用前加入缓冲液 A 中。(各类抑制剂的添加方法请按照抑制剂母液比例,例如母液是 100x,添加时按照 1:100 添加,即 1ml 缓冲液中添加 10ul 抑制剂)。
    4. 推荐使用 BCA 法测定蛋白浓度。

     

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      该产品被引用文献

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      ED-028 Minute™ 内体和细胞组分分离试剂盒
      ¥7300