- ¥2980
- LMAI Bio
- LM-0096R-FITC
- 中国/美国/欧洲
- 2025年07月11日
- ICC=1:50-200 IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Cow,
- Human, Mouse, Rat, Dog, Pig, Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-0096R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-alpha 1 Antitrypsin/FITC
- 抗体名：
Anti-alpha 1 Antitrypsin/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Cow,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
ICC=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
FITC标记的α-1抗胰蛋白酶抗体
| 英文名称 | Anti-alpha 1 Antitrypsin/FITC |
| 中文名称 | FITC标记的α-1抗胰蛋白酶抗体 |
| 别 名 | A1-Antitrypsin; Tryptase; Alpha-1-Antitrypsin; alpha 1-antitrypsin; A1A; A1AT; AAT; Alpha 1 antiproteinase; Alpha 1 protease inhibitor; alpha-1-AT; alpha1 PI; alpha1 proteinase inhibitor; Clade A (alpha 1 antiproteinase antitrypsin) member 1; MGC23330; MGC9222; PI; PI; PRO2275; Protease inhibitor 1 (anti elastase); Serpin A1; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1; SerpinA1; A1AT_HUMAN; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 免疫学 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human alpha 1 Antitrypsin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Alpha-1-antitrypsin is a protease inhibitor, deficiency of which is associated with emphysema and liver disease. The protein is encoded by a gene (PI) located on the distal long arm of chromosome 14. Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). Subcellular Location: Secreted. Short peptide from AAT: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Plasma. Post-translational modifications: N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. DISEASE: Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]. A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Similarity: Belongs to the serpin family. Database links: Entrez Gene: 5265 Human Entrez Gene: 24648 Rat Omim: 107400 Human SwissProt: P01009 Human SwissProt: P07758 Mouse SwissProt: P17475 Rat Unigene: 525557 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. α1-抗胰蛋白酶（A1AT）缺乏是最常见的遗传代谢病，能引起肺和肝的损伤。α1抗胰蛋白酶为呼吸系统的非特异性可溶因子，与呼吸道抵抗力关系密切，它可抑制多种酶的活性，包括细菌的酶，以及中性白细胞溶酶体分泌的蛋白酶、弹性蛋白酶、胶原酶、纤维蛋白溶酶和凝血酶。α1抗胰蛋白酶的缺乏与慢性阻塞性肺病的形成关系密切，因为它的缺乏，不能及时控制感染和炎症产生的多种蛋白酶，而造成肺组织破坏。 α1-抗胰蛋白酶由肝细胞产生,是一种分子量为45-56kDa的糖蛋白,它能抑制蛋白酶、弹性蛋白酶、胶原酶等多种水解酶的活性。 AAT也是一种糖蛋白，主要用于遗传性AAT缺乏症和良性恶性肝肿瘤、内胚窦瘤、组织细胞性淋巴瘤以及胰腺癌、胃癌、结肠癌等各种肿瘤的研究。此抗体与大、小鼠、狗和猪有交叉反应。 |
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文献和实验相关实验
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物，过葡萄糖凝胶G-25或G-50柱，分离出游离荧光素，收集标记的荧光抗体进行鉴定。
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FITC标记的α-1抗胰蛋白酶抗体
¥2980
