WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
50kDa
细胞定位
细胞浆 细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human ENPP7:351-458/458
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Function: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Note=Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex.
Tissue Specificity: Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
Post-translational modifications: N-glycosylated; required for activity and transport to the plasma membrane.
Similarity: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
SWISS: Q6UWV6
Gene ID: 339221
Database links:
Entrez Gene: 339221 Human
Entrez Gene: 238011 Mouse
Entrez Gene: 303729 Rat
SwissProt: Q6UWV6 Human
SwissProt: Q5EZ72 Rat
Unigene: 114084 Human
Unigene: 244114 Mouse
Unigene: 211790 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample: HT29 (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: FHC (human)Cell Lysate at 40 ug Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 48 kD