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100mL
RIPA裂解液
RIPA Lysis Buffer
本产品常温运输；保存于4℃，保质期12个月。
货号规格
goodPC101 RIPA 裂解液(高强度)goood100 mL
goodPC102 RIPA 裂解液(中强度)goood100 mL
goodPC103 RIPA 裂解液(弱强度)goood100 mL
goodPC104 RIPA 裂解液(强中弱套装)go50 mL×3
产品简介
goodRIPA裂解液(RIPA的本意是Radio Immunoprecipitation Assay)是一种传统的细胞组织快速裂解液，主要用于从动物细胞和组织中提取可溶性蛋白，其裂解得到的蛋白样品可以用于常规的Western Blot、IP及Elisa等实验。RIPA裂解液的配方有很多种，根据其裂解强度大致可以分为强、中、弱三类。用RIPA裂解液裂解得到的蛋白样品，可以使用 BCA蛋白定量试剂盒 (货号：ZJ101或ZJ102)测定蛋白浓度。
使用说明
◈ 对于悬浮细胞：
◈ 对于组织样品：
注意事项
产品选择参考
相关论文
good1. Jin Y, Qi G, Shou Y, et al. High throughput data-based, toxicity pathway-oriented development of a quantitative adverse outcome pathway network linking AHR activation to lung damages[J]. Journal of Hazardous Materials, 2022, 425: 128041.（IF 10.588）
good2. He, D., Wang, D., Lu, P., Yang, N., Xue, Z., Zhu, X., ... & Fan, G. (2020). Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene, 1-14. (IF 9.867)
good3. HE, Di, et al. Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene, 2021, 40.2: 355-368.(IF 9.867)
good4. Yang, Y., Zeng, B., Li, Y., Liang, H., Yang, Y., & Yuan, Q. (2020). Construction of MoS 2 field effect transistor sensor array for the detection of bladder cancer biomarkers. SCIENCE CHINA Chemistry.（IF 9.445）
good5. Ma R, Gong D, You H, et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland[J]. Cell Reports, 2022, 38(7): 110375. （IF 9.423）
good6. Yang, R., Chen, J., Xu, B., Yang, B., Fu, J., Xiao, S., ... & Wang, X. (2020). circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis. Molecular Therapy-Nucleic Acids, 22, 708-721. (IF 8.886)
good7. Lin S, Zhou L, Dong Y, et al. Alpha-(1, 6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling[J]. Cell death & disease, 2021, 12(12): 1-12.（IF 8.469）
good8. GAO, Xiaona, et al. Comparative effects of genistein and bisphenol A on non-alcoholic fatty liver disease in laying hens. Environmental Pollution, 2021, 117795. (IF 8.071)
good9. Hua T, Wang H, Fan X, et al. BRD4 Inhibition Attenuates Inflammatory Pain by Ameliorating NLRP3 Inflammasome-Induced Pyroptosis[J]. Frontiers in immunology, 2022, 13: 837977-837977.（IF 7.561）
good10. LI, Juehong, et al. Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult. Frontiers in immunology, 2021, 12: 1946.(IF 7.561)
good11. YU, Wenxia, et al. Harnessing A3G for efficient and selective C-to-T conversion at C-rich sequences. BMC biology, 2021, 19.1: 1-11. (IF 7.431)
good12. Xu X, Sui B, Liu X, et al. Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-кB pathway[J]. Materials Science and Engineering: C, 2021, 131: 112503.（IF 7.328）
good13. Cheng, B., Zhang, H., Hu, J., Peng, Y., Yang, J., Liao, X., ... & Lu, H. (2020). The immunotoxicity and neurobehavioral toxicity of zebrafish induced by famoxadone-cymoxanil. Chemosphere, 247, 125870. (IF 7.086)
good14. Cheng, B., Zou, L., Zhang, H., Cao, Z., Liao, X., Shen, T., ... & Lu, H. (2020). Effects of cyhalofop-butyl on the developmental toxicity and immunotoxicity in zebrafish (Danio rerio). Chemosphere, 263, 127849. (IF 7.086)
good15. CHENG, Bo, et al. Effects of cyhalofop-butyl on the developmental toxicity and immunotoxicity in zebrafish (Danio rerio). Chemosphere, 2021, 263: 127849. (IF 7.086)
good16. XIAO, Lili, et al. Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro. Frontiers in Cell and Developmental Biology, 2021, 9: 350.（IF 6.684）
good17. FU, Leming, et al. Autosomal recessive rod-cone dystrophy associated with compound heterozygous variants in ARL3 gene. Frontiers in cell and developmental biology, 2021, 9: 473.（IF 6.684）
good18. Zeng H, Li L, Gao Y, et al. Long noncoding RNA UCA1 regulates HCV replication and antiviral response via miR-145-5p/SOCS7/IFN pathway[J]. International Journal of Biological Sciences, 2021, 17(11): 2826.（IF 6.580）
good19. Yang, X. H., Zhang, B. L., Zhang, X. M., Tong, J. D., Gu, Y. H., Guo, L. L., & Jin, H. M. (2020). EGCG attenuates renal damage via reversing klotho hypermethylation in diabetic db/db mice and HK-2 cells. Oxidative medicine and cellular longevity, 2020.（IF 6.543）
good20. Liu, F., Chen, J. F., Wang, Y., Guo, L., Zhou, Q. M., Peng, C., & Xiong, L. (2019). Cytotoxicity of lanostane-type triterpenoids and ergosteroids isolated from Omphalia lapidescens on MDA-MB-231 and HGC-27 cells. Biomedicine & Pharmacotherapy, 118, 109273.（IF 6.529）
RIPA Lysis Buffer
本产品常温运输；保存于4℃，保质期12个月。
货号规格
goodPC101 RIPA 裂解液(高强度)goood100 mL
goodPC102 RIPA 裂解液(中强度)goood100 mL
goodPC103 RIPA 裂解液(弱强度)goood100 mL
goodPC104 RIPA 裂解液(强中弱套装)go50 mL×3
产品简介
goodRIPA裂解液(RIPA的本意是Radio Immunoprecipitation Assay)是一种传统的细胞组织快速裂解液，主要用于从动物细胞和组织中提取可溶性蛋白，其裂解得到的蛋白样品可以用于常规的Western Blot、IP及Elisa等实验。RIPA裂解液的配方有很多种，根据其裂解强度大致可以分为强、中、弱三类。用RIPA裂解液裂解得到的蛋白样品，可以使用 BCA蛋白定量试剂盒 (货号：ZJ101或ZJ102)测定蛋白浓度。
使用说明
good1. 取适当量的裂解液(每1×106个细胞需要约50~100 µL或每20 mg组织样本需要约150~250 μL)，在使用前数分钟内将 蛋白酶抑制剂 按1:100(V/V)加入其中( 蛋白酶抑制剂 需另行购买，货号：GRF101)；
good注意：如所需提取的为磷酸化蛋白，还需在RIPA裂解液中按1:100(V/V)加入 磷酸酶抑制剂 (货号：GRF102)。
good2. 样本裂解(需在冰上操作)：
◈ 对于贴壁细胞：
(1) 弃去培养基，用1×PBS(货号：PS110，或生理盐水、无血清培养液)洗一遍(如果血清中的蛋白对实 验没有干扰，也可以不洗)；
(2) 尽可能地弃去PBS(多余的液体将降低裂解液的浓度)；
(3) 按照每1×106个细胞需要约50~100 µL的比例加入RIPA裂解液，比如6孔板每孔细胞量大约需加入150~250 μL裂解液。用移液器吹打数下，使裂解液和细胞充分接触。通常裂解液接触细胞1~2 s后，细胞就会被裂解；
(4) 将所有液体转入新的离心管中；
◈ 对于悬浮细胞：
(1) 将细胞转移至离心管中，离心收集细胞，弃去培养基；
(2) 用1×PBS(货号：PS110，或生理盐水、无血清培养液)洗一遍(如果血清中的蛋白对实验没有干扰，也可以不洗)；
(3) 尽可能的弃去PBS(多余的液体将降低裂解液的浓度)；
(4) 按照每1×106个细胞需要约50~100 µL的比例加入RIPA裂解液，比如6孔板每孔细胞量大约需加入150~250 μL裂解液。用移液器吹打数下，使裂解液和细胞充分接触。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必须分装成5×105~1×106个细胞/管，然后再裂解；
◈ 对于组织样品：
(1) 把组织剪切成细小的碎片；
(2) 按照每20 mg组织样本加入150~250 μL裂解液的比例加入裂解液；
go注意：如果样本裂解不充分，可以适当提高裂解液的用量；若需要高浓度的蛋白样品，也可适当降低裂解液的用量。
(3) 用玻璃匀浆器匀浆，直至样本充分裂解；
go注意：若组织样本非常细小，可以适当剪切后直接加入裂解液，通过强烈涡旋振荡使其裂解充分。
3. 充分裂解后，10,000~14,000×g离心3~5 min，小心地将上清液(蛋白样品)移入新的离心管中，即可进行后续的PAGE凝胶电泳、Western Blot和免疫沉淀等操作。得到的蛋白样品可分装并长期保存于-80℃。
注意事项
good1. 4℃保存时，裂解液中的SDS易析出，使用前请置于37℃使其完全溶解，待恢复到室温即可使用；
good2. 裂解样品的所有步骤都需在冰上或4℃进行；
good3. RIPA裂解液的裂解产物中经常会出现一小团透明胶状物，此为正常现象，该物质为基因组DNA等形成的复合物。如不检测和基因组DNA紧密结合的蛋白，可以直接离心取上清用于后续实验；若需要检测此类蛋白，则可以通过超声处理打散该透明胶状物，随后离心取上清即可用于后续实验。但如果检测一些常见的转录因子，例如NF-kappaB、p53等，通常不必进行超声处理就可完成检测；
good4. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
good5. 本产品仅限科研使用。
产品选择参考
相关论文
good1. Jin Y, Qi G, Shou Y, et al. High throughput data-based, toxicity pathway-oriented development of a quantitative adverse outcome pathway network linking AHR activation to lung damages[J]. Journal of Hazardous Materials, 2022, 425: 128041.（IF 10.588）
good2. He, D., Wang, D., Lu, P., Yang, N., Xue, Z., Zhu, X., ... & Fan, G. (2020). Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene, 1-14. (IF 9.867)
good3. HE, Di, et al. Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene, 2021, 40.2: 355-368.(IF 9.867)
good4. Yang, Y., Zeng, B., Li, Y., Liang, H., Yang, Y., & Yuan, Q. (2020). Construction of MoS 2 field effect transistor sensor array for the detection of bladder cancer biomarkers. SCIENCE CHINA Chemistry.（IF 9.445）
good5. Ma R, Gong D, You H, et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland[J]. Cell Reports, 2022, 38(7): 110375. （IF 9.423）
good6. Yang, R., Chen, J., Xu, B., Yang, B., Fu, J., Xiao, S., ... & Wang, X. (2020). circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis. Molecular Therapy-Nucleic Acids, 22, 708-721. (IF 8.886)
good7. Lin S, Zhou L, Dong Y, et al. Alpha-(1, 6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling[J]. Cell death & disease, 2021, 12(12): 1-12.（IF 8.469）
good8. GAO, Xiaona, et al. Comparative effects of genistein and bisphenol A on non-alcoholic fatty liver disease in laying hens. Environmental Pollution, 2021, 117795. (IF 8.071)
good9. Hua T, Wang H, Fan X, et al. BRD4 Inhibition Attenuates Inflammatory Pain by Ameliorating NLRP3 Inflammasome-Induced Pyroptosis[J]. Frontiers in immunology, 2022, 13: 837977-837977.（IF 7.561）
good10. LI, Juehong, et al. Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult. Frontiers in immunology, 2021, 12: 1946.(IF 7.561)
good11. YU, Wenxia, et al. Harnessing A3G for efficient and selective C-to-T conversion at C-rich sequences. BMC biology, 2021, 19.1: 1-11. (IF 7.431)
good12. Xu X, Sui B, Liu X, et al. Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-кB pathway[J]. Materials Science and Engineering: C, 2021, 131: 112503.（IF 7.328）
good13. Cheng, B., Zhang, H., Hu, J., Peng, Y., Yang, J., Liao, X., ... & Lu, H. (2020). The immunotoxicity and neurobehavioral toxicity of zebrafish induced by famoxadone-cymoxanil. Chemosphere, 247, 125870. (IF 7.086)
good14. Cheng, B., Zou, L., Zhang, H., Cao, Z., Liao, X., Shen, T., ... & Lu, H. (2020). Effects of cyhalofop-butyl on the developmental toxicity and immunotoxicity in zebrafish (Danio rerio). Chemosphere, 263, 127849. (IF 7.086)
good15. CHENG, Bo, et al. Effects of cyhalofop-butyl on the developmental toxicity and immunotoxicity in zebrafish (Danio rerio). Chemosphere, 2021, 263: 127849. (IF 7.086)
good16. XIAO, Lili, et al. Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro. Frontiers in Cell and Developmental Biology, 2021, 9: 350.（IF 6.684）
good17. FU, Leming, et al. Autosomal recessive rod-cone dystrophy associated with compound heterozygous variants in ARL3 gene. Frontiers in cell and developmental biology, 2021, 9: 473.（IF 6.684）
good18. Zeng H, Li L, Gao Y, et al. Long noncoding RNA UCA1 regulates HCV replication and antiviral response via miR-145-5p/SOCS7/IFN pathway[J]. International Journal of Biological Sciences, 2021, 17(11): 2826.（IF 6.580）
good19. Yang, X. H., Zhang, B. L., Zhang, X. M., Tong, J. D., Gu, Y. H., Guo, L. L., & Jin, H. M. (2020). EGCG attenuates renal damage via reversing klotho hypermethylation in diabetic db/db mice and HK-2 cells. Oxidative medicine and cellular longevity, 2020.（IF 6.543）
good20. Liu, F., Chen, J. F., Wang, Y., Guo, L., Zhou, Q. M., Peng, C., & Xiong, L. (2019). Cytotoxicity of lanostane-type triterpenoids and ergosteroids isolated from Omphalia lapidescens on MDA-MB-231 and HGC-27 cells. Biomedicine & Pharmacotherapy, 118, 109273.（IF 6.529）
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PC102 RIPA 裂解液（中强度）
￥168
