- ￥6022.06
- Swant
- 瑞士
- CB-38a (new batch)-200 µl
- 2025年07月13日
- Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments.
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Make small portions (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
- 克隆性：
多克隆
- 适应物种：
Rabbit
- 供应商：
上海联迈生物工程有限公司
- 应用范围：
Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments.
- 靶点：
Calbindin D-28k
- 规格：
200 µl
研究领域：Calbindin
存储溶液：Lyophilized antiserum (no preservatives).
参考文献：1. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
应用：Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method.Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments.
官网链接：https://www.swant.com/pdfs/Rabbit_anti_calbindin_D-28k_CB38.pdf
商品关键词：Rabbit anti-Calbindin D-28k ,CB-38a (new batch)-200 µl ,Swant
简单描述：This antiserum was produced against recombinant rat calbindin D-28k (CB). It crossreacts with calbindin D-28k from many other species, including human, monkey, rat, mouse chicken and fish (Fig. 1). In immunoblots it recognizes a single band of approximately 27 -28 kDa. In samples with very high (10-fold higher than CB) concentrations of the related protein calretinin, a larger band (29 - 30 kDa) may appear in Western blots. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium-binding protein could be also revealed. In case of doubt, we suggest to switch to Swant’s monoclonal antibody 300 directed against calbindin D-28k, which does not recognize calretinin.
Lot No: 9.03Reconstitution: with 200 µl bidistilled water.
Immunoblot: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB 38 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. chicken. In all species, only a band at 28 KDa is detected.
Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum CB38 immunolabels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of calretinin knock out mice (Fig. 2b).
Product: Antiserum
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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