• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构：
    • 成为企业机构
    • 个人用户：
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Immunofluorescence: Rabbit Anti-Murine RELMα

        互联网

        2005

        实验步骤

         

        The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections.

        1. Deparaffinize and rehydrate the tissue section.

        2. Perform heat-induced antigen retrieval by boiling the tissue section in 10mM pH 6.0 citrate buffer for 25 minutes.

        3. Incubate the tissue section with blocking buffer for 20 minutes.

        4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide twice for three minutes (1X PBS/0.05% Tween 20).

        5. Incubate the tissue section with a fluorescent conjugated secondary antibody for 2 hours at room temperature. Wash the slide twice for three minutes.

        6. Counterstain the tissue section with DAPI.

        The following protocol used B6 mice lung sections that were injected with helminth Schistosoma mansoni eggs. Inflamed lung sections were fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections. Some tissue sections or cell preparations stained were from day 14 bleomycin-instilled mice.

        1. Deparaffinize and rehydrate the tissue section.

        2. Perform heat-induced antigen retrieval by boiling the tissue section in 10mM pH 6.0 citrate buffer for 25 minutes.

        3. Incubate the tissue section with blocking buffer for 20 minutes.

        4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 0.2 μg/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide twice for three minutes (1X PBS/0.05% Tween 20).

        5. Incubate the tissue section with a fluorescent conjugated secondary antibody for 2 hours at room temperature. Wash the slide twice for three minutes.

        6. Counterstain the tissue section with DAPI.

        ad image
        ad image

        相关产品推荐

        Anti-H7N9 Hemagglutinin / HA 抗体, 血凝活性, 兔单抗

        Anti-H7N9 Hemagglutinin / HA 抗体, 血凝活性, 兔单抗

        ￥2500

        Retnla | RELM alpha Rabbit pAb(bs-1884R)-50ul/100ul/200ul

        Retnla | RELM alpha Rabbit pAb(bs-1884R)-50ul/100ul/200ul

        ￥1180

        Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use)，ExactAb™, 已验证, 见COA，阿拉丁

        Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use)，ExactAb™, 已验证, 见COA，阿拉丁

        ¥74.90

        Recombinant Murine minute virus/MVM NS1 Protein (N-His Tag), 重组Murineminute virus/MVM NS1蛋白 (N端His标签)

        Recombinant Murine minute virus/MVM NS1 Protein (N-His Tag), 重组Murineminute virus/MVM NS1蛋白 (N端His标签)

        ￥1268

        抗Anti-H7N9 Hemagglutinin / HA 抗体, 兔多抗, 抗原亲和纯化

        抗Anti-H7N9 Hemagglutinin / HA 抗体, 兔多抗, 抗原亲和纯化

        ￥800

        相关问答

        问

        二抗anti-mouse可以代替anti-rat吗？

        3 回答 4194 围观
        2023-07-12

        问

        RABBIT ANTI HUMAN BETA AMYLOID (aa1-42)抗体做Elisa实验板的板包被，需要多少浓度的抗体

        4 回答 2183 围观
        2023-07-07

        问

        Anti-KAT5 / Tip60抗体

        1 回答 1167 围观
        2023-03-30

        相关方法

        🔥 免疫荧光（Immunofluorescence, IF）

        赤霉素对α-淀粉诱导合成的影响

        α 突触核蛋白操作指南

        推荐阅读

        Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining

        Teratology Studies in the Rabbit

        Rabbit Whole Embryo Culture

        无忧采购 轻松科研

        无忧采购 轻松科研

        提问
        扫一扫
        丁香实验小程序二维码
        领取干货资料
        反馈
        TOP
        打开小程序