Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.
靶点:
Calbindin D-28k
规格:
1 ml
商品分类:Antibodies against Ca2+ -bindin
研究领域:Calbindin
存储溶液:Lyophilized concentrated supernatant (no preservatives).
应用:Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method.We recommend that the optimal dilutions be determined by titration experiments.
商品关键词:Monoclonal anti Calbindin D-28k,300-1 ml ,Swant
简单描述:Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay.The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C.McAB 300 reacts specifically with calbindin D-28k on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (but probably not fish, Fig. 1). McAB 300 does not cross-react with calretinin or other known calcium binding-proteins. McAB 300 specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000, IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detectscalbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 1012 L/M. McAB 300 against calbindin D-28k specifically localizes calbindin using free-floating or mounted sections of brain, kidney and pancreas of probably all vertebrates with the exception of fishes.
Lot No: 07 (F)Reconstitution: with 200 µl / 500 µl or 1 ml bidistilled water.
Immunoblot of brain extracts: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected.
Immunohistochemistry on Calbindin D-28k knock-out mice: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected.