- ￥23933.82
- Swant
- 瑞士
- 300-1 ml
- 2025年07月11日
- Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.
- Mouse monoclonal, IgG1
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
- 克隆性：
多克隆
- 适应物种：
Mouse monoclonal, IgG1
- 供应商：
上海联迈生物工程有限公司
- 应用范围：
Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.
- 靶点：
Calbindin D-28k
- 规格：
1 ml
研究领域：Calbindin
存储溶液：Lyophilized concentrated supernatant (no preservatives).
参考文献：1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
应用：Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method.We recommend that the optimal dilutions be determined by titration experiments.
官网链接：https://www.swant.com/pdfs/Monoclonal_anti_calbindin_d28k_300.pdf
商品关键词：Monoclonal anti Calbindin D-28k,300-1 ml ,Swant
简单描述：Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay.The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C.McAB 300 reacts specifically with calbindin D-28k on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (but probably not fish, Fig. 1). McAB 300 does not cross-react with calretinin or other known calcium binding-proteins. McAB 300 specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000, IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detectscalbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 1012 L/M. McAB 300 against calbindin D-28k specifically localizes calbindin using free-floating or mounted sections of brain, kidney and pancreas of probably all vertebrates with the exception of fishes.
Lot No: 07 (F)Reconstitution: with 200 µl / 500 µl or 1 ml bidistilled water.
Immunoblot of brain extracts: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected.
Immunohistochemistry on Calbindin D-28k knock-out mice: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected.
Product: Conc. supernat.
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文献和实验monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
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