CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞 的详细介绍
CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞
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CRL-1592™ |
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IEC-6 |
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A Quaroni |
| Biosafety Level: |
1 |
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frozen |
| Medium & Serum: |
See Propagation |
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adherent |
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Rattus norvegicus (rat) |
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epithelial |
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Organ: small intestine
Tissue: epithelium
Disease: normal |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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transfection host (Roche FuGENE® Transfection Reagents) |
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male |
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ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
Temperature: 37.0°C |
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Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week |
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Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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