SW1116 结肠腺癌细胞

SW1116 结肠腺癌细胞

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  • SW1116
  • 2025年12月19日
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    CCL-233 SW1116 人结肠腺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和养条件！

    CCL-233 SW1116 人结肠腺癌细胞 的详细介绍
    CCL-233 SW1116 人结肠腺癌细胞
    ATCC® Number: CCL-233™
    Designations: SW1116 [SW 1116, SW-1116]
    Depositors: A Leibovitz
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    CCL-233 SW1116 人结肠腺癌细胞
    :
    Cytogenetic Analysis: modal number = 60; range = 50 to 62
    The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells.
    Ethnicity: Caucasian
    Comments: CSAp negative (CSAp-).
    Colon antigen 3, negative.
    The cells are positive for keratin by immunoperoxidase staining.
    The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.
    N-myc and N-ras oncogene expression were not detected.
    Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Cells must be subcultured at about 80% confuency , before they reach 90%.
    Protocol: 1. Remove and discard culture medium.

    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

    5. Add appropriate aliquots of the cell suspension to new culture vessels.

    6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 1 to 2 times per week
    : CCL-233 SW1116 人结肠腺癌细胞
    CCL-233 SW1116 人结肠腺癌细胞

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