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- 文献和实验
- 技术资料
- 供应商:
ATCC
- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 相关疾病:
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- 免疫类型:
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- 细胞形态:
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- 是否是肿瘤细胞:
是
- 运输方式:
常温
- 年限:
2026
- 生长状态:
正常
- 库存:
大量
CRL-2632 Pfeiffer 弥漫性大细胞淋巴瘤B淋巴细胞,ATCC 细胞|细胞系|细胞株|细胞,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件
| ATCC® Number: | CRL-2632™ | Price: | $379.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Designations: Pfeiffer
Depositors: H Ben-Bassat
Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Antigen Expression: CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile (STR): CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
Age: adult adult
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei. [53289]
The cells are negative for Epstein-Barr virus. [53289]
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
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文献和实验[1]由细菌所免疫的动物体液中存在的抗体,在补体存在下破坏相应细菌菌体的现象称为溶菌作用。 1894年 R. F. J. Pfeiffer发现,如果在经痢疾菌免疫的豚鼠腹腔内注射痢疾菌,则该细菌发生特异的裂解,并已将这种作用用于细菌鉴定中( Pfeiffer现象或 Pfeiffer溶菌现象)。如加入健康动物的新鲜血清作为补体,则溶菌现象也可在试管中发生。此称为 Neisser- Wechsberg试管溶菌反应。 [2]指由于噬菌体在细菌细胞内增殖,形成溶解细菌细胞壁的酶,这种酶可使细菌
Approximately 100 yr ago Richard Pfeiffer, a co-worker of Robert Koch in Berlin, discovered that cholera bacteria produced, in addition to heat-labile exotoxin, another toxin (1 ). In contrast to the secreted exotoxins this new, heat-stable
The Pathogenesis of Disease Due to Type b Haemophilus influenzae
Haemophilus influenzae is a Gram-negative bacterium that was first described by Pfeiffer in 1892 (1 ). This ubiquitous, human-specific organism was originally thought to be the etiologic agent of “influenza.” However, H. influenzae
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