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Pfeiffer

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  • 2025年12月18日
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    • 细胞类型

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    • 是否是肿瘤细胞

    • 运输方式

      常温

    • 年限

      2026

    • 生长状态

      正常

    • 库存

      大量

    CRL-2632 Pfeiffer 弥漫性大细胞淋巴瘤B淋巴细胞,ATCC 细胞|细胞系|细胞株|细胞,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

    CRL-2632 Pfeiffer 人弥漫性大细胞淋巴瘤B淋巴细胞 的详细介绍
    CRL-2632 Pfeiffer 人弥漫性大细胞淋巴瘤B淋巴细胞
    ATCC® Number: CRL-2632™ Price: $379.00
    Designations: Pfeiffer
    Depositors: H Ben-Bassat
    Isotype: IgG kappa
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: suspension
    Organism: Homo sapiens (human)
    Morphology: lymphoblast
    Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
    Derived from metastatic site: pleural effusion
    Cell Type: lymphoblast B lymphocyte;
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1992
    Antigen Expression: CD10; Homo sapiens
    CD19; Homo sapiens
    CD20; Homo sapiens
    CD38; Homo sapiens
    CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
    DNA Profile (STR): CSF1PO: 10, 11
    D13S317: 11, 12
    D16S539: 12
    D5S818: 10, 13
    D7S820: 8, 12
    THO1: 9, 9.3
    TPOX: 9
    vWA: 17, 18
    Amelogenin: XY
    Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
    Age: adult adult
    Gender: male
    Ethnicity: White
    Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei. [53289]
    The cells are negative for Epstein-Barr virus. [53289]
    This cell line is a model system for studying non-Hodgkin lymphomas.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Doubling Time: 24 to 30 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
    ATCC® Number: CRL-2632™ Price: $379.00
    Designations: Pfeiffer
    Depositors: H Ben-Bassat
    Isotype: IgG kappa
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: suspension
    Organism: Homo sapiens (human)
    Morphology: lymphoblast


    Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
    Derived from metastatic site: pleural effusion
    Cell Type: lymphoblast B lymphocyte;
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

    Isolation: Isolation date: 1992
    Antigen Expression: CD10; Homo sapiens
    CD19; Homo sapiens
    CD20; Homo sapiens
    CD38; Homo sapiens
    CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
    DNA Profile (STR): CSF1PO: 10, 11
    D13S317: 11, 12
    D16S539: 12
    D5S818: 10, 13
    D7S820: 8, 12
    THO1: 9, 9.3
    TPOX: 9
    vWA: 17, 18
    Amelogenin: XY
    Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
    Age: adult adult
    Gender: male
    Ethnicity: White
    Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei. [53289]
    The cells are negative for Epstein-Barr virus. [53289]
    This cell line is a model system for studying non-Hodgkin lymphomas.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Doubling Time: 24 to 30 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273


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      [1]由细菌所免疫的动物体液中存在的抗体,在补体存在下破坏相应细菌菌体的现象称为溶菌作用。 1894年 R. F. J. Pfeiffer发现,如果在经痢疾菌免疫的豚鼠腹腔内注射痢疾菌,则该细菌发生特异的裂解,并已将这种作用用于细菌鉴定中( Pfeiffer现象或 Pfeiffer溶菌现象)。如加入健康动物的新鲜血清作为补体,则溶菌现象也可在试管中发生。此称为 Neisser- Wechsberg试管溶菌反应。 [2]指由于噬菌体在细菌细胞内增殖,形成溶解细菌细胞壁的酶,这种酶可使细菌

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