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文献和实验Preparation of 2′‐Deoxy‐2′‐Methylseleno‐Modified Phosphoramidites and RNA
. Ennifar, E., Carpentier, P., Ferrer, J.L., Walter, P., and Dumas, P. 2002. X‐ray‐induced debromination of nucleic acids at the Br K absorption edge and implications for MAD phasing. Acta Crystallogr. D Biol
Modified Eberwine (antisense) RNA Amplification Protocol
, Pharmacia #27-2035-01) 1 uL E. coli DNA Ligase (10 U/uL, NEB #205L) 4 uL E. coli DNA Polymerase I--holoenzyme (10 U/uL, NEB #209L) 1 uL RNase H (2 U/uL, GibcoBRL #18021-071) Incubate 16C for 2 hours. If amplifying from mRNA, stop reaction with 10
A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins
is the need to discriminate modified proteins, usually occurring at substoichiometric levels, from the large amount of nonmodified proteins. Moreover, precise localization of the nitration site is often required to fully describe the biological process. Identification
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