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- Cellgro
- 91-200-77
- 2025年09月21日
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1 x 20L
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文献和实验AMNIOTIC FLUID CULTURES ON COVERSLIPS
the following day. One coverslip should be reserved from harvest for possible subculturing. (If, on day 6, coverslips are not ready for a full media change, carefully aspirate off 1 ml of media per coverslip, discard, and refeed with 1 ml fresh media using
CCMB buffer to make chemical competent cell
on ice 20 minutes Centrifuge again at 4C and resuspend in 20 ml of ice cold CCMB80 buffer. Incubate on ice for 20 minutes Aliquot to chilled screw top 2 ml vials or 50 μl into chilled microtiter plates Store at -80C indefinitely. Flash freezing
TOP10 chemically competent cells
10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) 80 mM CaCl2 .2H2 O (11.8 g/L) 20 mM MnCl2 .4H2 O (4.0 g/L) 10 mM MgCl2 .6H2 O (2.0 g/L) 10% glycerol (100 ml/L) adjust pH DOWN to 6.4 with 0.1N HCl if necessary
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