β-Galactose Dehydrogenase

from Pseudomonas fluorescens

General description

D-galactose:NAD⁺ 1-oxidoreductase

Specificity

Galactose dehydrogenase will oxidize D-galactose [K_m = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (K_m = 0.24 mM; relative rate = 1.0) or NADP (K_m = 2.3 mM; relative rate = 0.32).

Quality

Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH

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