该产品由Abcam公司Expedeon生产,之前被称为Lightning Link®碱性磷酸酶标记试剂盒。702-0005与100µg尺寸相同。702-0010与3 x 100 ug尺寸相同。702-0030与3 x 10 ug尺寸相同。702-0015与1 mg尺寸相同。
Kit size
Recommended maximum amount of antibody
Maximum antibody volume1
3 x10 µg
3 x 10 µg
3 x 10 µL
100 µg
1 x 100 µg
1 x 100 µL
3 x 100 µg
3 x 100 µg
3 x 100 µL
1 mg
1 x 1 mg
1 x 1 mL
Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1
0.1% BSA
50% glycerol
0.1% sodium azide
PBS
Potassium phosphate
Sodium chloride
HEPES
Sucrose
Sodium citrate
EDTA
Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%