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- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX128145
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
0.09 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ATR (phospho Thr1989)
- 抗体英文名:
ATR (phospho Thr1989) antibody
- 抗体名:
ATR (phospho Thr1989) 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody (GTX128145).
Western blot analysis was performed using ATR (phospho Thr1989) antibody (GTX128145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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