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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, E. coli, Mosquito, Nematode, Pika, Plant, Fish, Bacteria, Insect, milkfish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100118
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
0.11 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GAPDH
- 抗体英文名:
GAPDH antibody
- 抗体名:
GAPDH 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
GAPDH antibody detects GAPDH protein at endoplasmic reticulum, cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.
Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118).
Western blot analysis was performed using GAPDH antibody (GTX100118).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
,但是进口品牌如Abcam、Millipore、CST等价格偏高,一般在¥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体,分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody (货号E021010 )是小鼠源的单抗,其效价很高,厂商推荐的WB起始稀释比率为1:1000,实际应用过程中,有使用1:10000或更高的报告,远远高于此稀释比率,说明其免疫效价很高,另外,其检测种属也很多,包括人、牛、猪、小鼠
【信息】Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体)招代理
Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体) 背景 甘油醛 -3- 磷 酸脱氢酶( Glyceraldehyde 3-phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶,分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的,常用作 Western Blot 实验的内参照. 来源 兔抗
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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