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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9514R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
PI14
- 抗体名:
蛋白酶抑制剂14抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Horse,
- 免疫原:
KLH conjugated synthetic peptide derived from human SERPINI2/PI14:151-250/405
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | PI14 |
| 中文名称 | 蛋白酶抑制剂14抗体 |
| 别 名 | MEPI; Myoepithelium derived serine protease inhibitor; Pancpin; Pancreas specific protein TSA2004; Peptidase inhibitor 14; PI 14; PI14; Protease inhibitor 14; Serine (or cysteine) proteinase inhibitor clade I (neuroserpin) member 2; Serine (or cysteine) proteinase inhibitor clade I (pancpin) member 2; Serpin I2; Serpin peptidase inhibitor clade I (pancpin) member 2; TSA2004; SPI2_HUMAN; SERPINI2. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Horse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SERPINI2/PI14:151-250/405 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: SERPINI2 is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes, including coagulation, fibrinolysis, development, malignancy and inflammation. It may have a role in a growth-control, possibly growth-suppressing pathway and, when impaired, may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family, a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. Subcellular Location: Secreted Tissue Specificity: Expressed in pancreas and adipose tissues. Similarity: Belongs to the serpin family. SWISS: O75830 Gene ID: 5276 Database links: Entrez Gene: 5276 Human Entrez Gene: 67931 Mouse Entrez Gene: 171149 Rat Omim: 605587 Human SwissProt: O75830 Human SwissProt: Q9JK88 Mouse Unigene: 445555 Human Unigene: 259446 Mouse Unigene: 54500 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验核蛋白提取 实验准备: 1 4℃离心机 2 根据标本数计算所需I,II,III,IV总体积,取出液体,并加入蛋白酶抑制剂,(III由加蛋白酶抑制剂I与加蛋白酶抑制剂II混合物) 实验操作: 1 收集细胞。10ml 对照细胞(1-2*105/ml )培养48h后,15ml 离心管收集,300g(1370 rpm)* 5min。弃上清,加1ml 1*PBS轻轻涡旋混匀后转移至1.5ml EP管。 2 4℃,300g * 5min,弃上清。 3 重悬
time PCR 之前都要把 sample 离心保证取样的准确。 11、1~10ul 的*取 3ul 以上才比较准确,所以考虑好自己 PCR 反应体系的配置。 12、一个 ChIP 一般需要 3~4 天的时间,其中有几个步骤是可以停下来的: 1)细胞收集:用含蛋白酶抑制剂的 PBS 洗涤离心,去上清的细胞收集液可置 -80° 冻存; 2)用 SDS 重悬细胞后可置 -80° 冻存; 3)sonication 结束,离心后的上清置新的离心管后可 -80° 冻存; 4)reverse cross
signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好,或者抗体不好,不知道还有没有可能是没有加磷酸化酶抑制剂,以至于磷酸化的蛋白被去磷酸化了? 谢谢啦~ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功? 用PLB裂解细胞得到的蛋白适合做REPORTER,做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
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