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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-6909R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
BMP9
- 抗体名:
骨形态发生蛋白9抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human BMP9:351-429/429
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | BMP9 |
| 中文名称 | 骨形态发生蛋白9抗体 |
| 别 名 | BMP 9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2; GDF-2; growth differentiation factor 2; GDF2_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 细胞周期蛋白 转录调节因子 细胞分化 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12/47kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP9:351-429/429 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. [provided by RefSeq, Jul 2008]. Function: Could be involved in bone formation. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: Q9UK05 Gene ID: 2658 Database links: Entrez Gene: 2658 Human Entrez Gene: 12165 Mouse Entrez Gene: 290921 Rat Omim: 605120 Human SwissProt: Q9UK05 Human SwissProt: Q9WV56 Mouse Unigene: 218418 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12/47 kD Observed band size: 50 kD ![]() Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12/47 kD Observed band size: 50 kD ![]() Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP9) Polyclonal Antibody, Unconjugated (bs-6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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文献和实验baifeng1979 请教各位实验高手,我在做重组人骨形态发生蛋白2(rhBMP-2)的体内缓释实验,实验过程是将复合有rhBMP-2的载体材料植入小鼠四肢肌肉内,定期测定体内rhBMP-2的缓释和表达水平,请问采用什么方法更准确、简便,当初想采用ELISA定期测定小鼠外周血清,但有人说小鼠该蛋白与人有较强的同源性,测定值包含有自身BMP,那么如何将内源性和外源性区别开呢。另外,复合rhBMP-2载体材料植入区软组织的该蛋白的表达和定量用组织匀浆ELISA法
and mitophagy in lung fibroblasts 的研究论文。 该研究表明,骨形态发生蛋白 4 (BMP4) 在 人肺中的下调,在 IPF 疾病发生和进展中起 关键作用,BMP4 信号通路通过调节肺成 纤维细胞的衰老和线粒体自噬抑制肺纤维 化。因此,BMP4 信号的激活可能是治疗 IPF 肺纤维化的一种新型有效的治疗方法。 血清型:AAV9 注射方式:气管内注射 病毒滴度:1x1010 vp/ml 靶向部位:肺 动物模型:C57BL/6 BMP4+/+ and BMP4+/- mice
liushuitao 比如 骨形态发生蛋白BMP、转化生长因子-β1 TGF-β1 、骨钙素 OCN 、碱性磷酸酶ALP等, 已知这些蛋白,怎么从文库中找到与其对应的RNA或DNA呢? 小弟学临床的,对这些了解较少,现写标书要用,望各位大侠赐教。找的方法步骤越详细越好,不甚感激!:) zhujoker 建议借一本生物信息学的书,比如 生物信息学与功能基因组学 = Bioinformatics
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