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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Bme1390I (ScrFI) (10 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实在有限公司
- 保存条件：
-20℃
- 规格：
500units
Bme1390I (ScrFI) (10 U/µL)
Enzyme : Bme1390I (ScrFI)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer O
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : CpG methylation-sensitive, Not dam methylation-sensitive, dcm methylation-sensitive
5' C C ↓ N G G 3'
3' G G N ↑ C C 5'
Thermo Scientific Bme1390I (ScrFI) restriction enzyme recognizes CC^NGG sites and cuts best at 37°C in O buffer (isoschizomers: BmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: Assayed using lambda DNA (dcm-) (#SD0021). Bme1390I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163. For methylation sensitivity, refer to product specifications.
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply
Protein Expression and Purification of MBP-T antigen (131-259)
elutes as a single sharp peak.Collect the fraction and freeze the protein at -80 ℃ until further use. Buffer A 50 mM HEPES,0.3 M NaCl,1 mM BME,10 mM Imidazole,1 tablet PI/L,pH 7.5 Buffer B 50 mM HEPES,0.3 M NaCl,1 mM BME,300 mM Imidazole,1 tablet PI
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