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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
HpyF10VI (MwoI) (10 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实在有限公司
- 保存条件：
-20℃
- 规格：
300units
HpyF10VI (MwoI) (10 U/µL)
Enzyme : HpyF10VI (MwoI)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer Tango
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive
5' G C N N N N N ↓ N N G C 3'
3' C G N N ↑ N N N N N C G 5'
Thermo Scientific HpyF10VI (MwoI) restriction enzyme recognizes GCNNNNN^NNGC sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: BstMWI, MwoI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
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文献和实验人VI型胶原蛋白(Collagen VI)ELISA试剂盒 说明书
为横坐标， OD 值为纵坐标，在坐标纸上作图，画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 Collagen VI 含量。 试剂盒性能 1. 灵敏度 ： 最小的Collagen VI 检测浓度小于 10n g/ml。 2. 特异性： 可同时检测重组或天然的人 Collagen VI 。 不与 人其它细胞因子有交叉反应。 3. 重复性：板内、板见变异系数均小于10％。 注意事项
(1992) The oligonucleotides we use are kits, contaning 20 decamers, from Operon Technologies Inc. . I. DNase I treatment of RNA mix and incubate for 30 min. at 37 °C : 50 µgRNA, 10 µl RNase inhibitor (1 U/µl), 1 µl
Construction and Characterization of Adenovirus Vectors
2006 ). Suspend the DNA in a total volume of 25 µL of 0.1X TE-Ad. 12. Repeat Steps 5-10 with 5 µL of DNA (from Step 11) and 100 µL of DH5 cells, rather than BJ5183. 13. Select two colonies from each plate. Purify the DNA
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