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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
有效期2年
- 英文名:
NTP Set (ATP, CTP, UTP, GTP, 100 mM each)
- 库存:
大量
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
1Set(4vial)
Product Information
| Product Name |
Cat # |
Size |
Price (¥) |
| NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each) |
10133ES03 |
1 Set (4 vials) |
2453 |
Product Description
The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes.
This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.
Product Properties

| Component Number |
Components |
Size |
| 10133-A |
ATP (100 mM) |
1 mL |
| 10133-B |
UTP (100 mM) |
1 mL |
| 10133-C |
CTP (100 mM) |
1 mL |
| 10133-D |
GTP (100 mM) |
1 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.
Cautions
1. Keep on ice while using and return immediately to -20°C after use.
2. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB220514
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文献和实验polymerase as appropriate 2. Mix then spin down briefly. 3. Incubate at 37'C for 2-3 hours. 4. Add 2 ul DNase I, RNase free to remove DNA template. 5. Incubate at 37'C for 15 minutes. 10X NTP Labeling Mix: 10mM ATP, CTP, GTP, 6.5mM UTP, 3.5mM DIG in Tris
for 15 minutes. 10X NTP Labeling Mix: 10mM ATP, CTP, GTP, 6.5mM UTP, 3.5mM DIG in Tris-HCl, pH 7.5. 10X Transcription Buffer: 400mM Tris-HCl, pH 8.0; 60mM MgCl2, 100mM dithioerythritol, 20mM spermidine, 100mM NaCl, 1U/ml RNase Inhibitor
核苷的磷酸酯,是核酸的结构单元。分成核苷酸和脱氧核苷酸两大类。核苷的磷酸酯叫核苷酸,脱氧核苷的磷酸酯叫脱氧核苷酸。在细胞代谢中起作用的是5′-核苷酸,即磷酸基连在5′碳原子的羟基上。 核苷酸的5'磷酸基上再联结一个磷酸基即成各种核苷二磷酸(NDP或dNDP),核苷二磷酸的磷酸基上再联结一个磷酸基即成各种核苷三磷酸(NTP或dNTP),其中核苷三磷酸化合物最重要,ATP、UTP、CTP和GTP是RNA合成的前体;dGTP、dCTP







