HYB 021-08 is specific for human trypsin, trypsinogen and trypsin in complex with a1-antitrypsin.
Immunogen
Trypsin purified from human pancreatic tissue
Application
ELISA, WB
Species reactivity (positive)
Human, bovine
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
5644
Clone number
2C4
Epitope
Epitope differs from that of HYB 021-03
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Trypsin is a 24 kDa enzyme and is a member of the serine proteinase family. It is produced in the pancreas as an inactive precursor trypsinogen. The active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin predominantly cleaves proteins at the carboxyl side of the amino acids lysine and arginine. A common trypsin substrate is chymotrypsinogen which is cleaved to produce active chymotrypsin.
High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.
Application 1
ELISA HYB 021-08 reacts with human trypsin coated directly onto a microtiter plate and is suitable as a detection antibody in sandwich ELISA with a coat of HYB 021-03
Application 2
WB HYB 021-08 recognizes human trypsin in Western blotting under reducing conditions.