Anti-Trypsin (human)

Anti-Trypsin (human)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
官方网站：www.bioporto.com

Cat. No.

HYB 021-08

Product name

Anti-Trypsin (human)

Description

Mouse monoclonal antibody

Specificity

HYB 021-08 is specific for human trypsin, trypsinogen and trypsin in complex with a1-antitrypsin. 

Immunogen

Trypsin purified from human pancreatic tissue

Application

ELISA, WB

Species reactivity (positive)

Human, bovine

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

5644

Clone number

2C4

Epitope

Epitope differs from that of HYB 021-03

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Trypsin is a 24 kDa enzyme and is a member of the serine proteinase family. It is produced in the pancreas as an inactive precursor trypsinogen. The active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin predominantly cleaves proteins at the carboxyl side of the amino acids lysine and arginine. A common trypsin substrate is chymotrypsinogen which is cleaved to produce active chymotrypsin.
High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.

Application 1

ELISA
HYB 021-08 reacts with human trypsin coated directly onto a microtiter plate and is suitable as a detection antibody in sandwich ELISA with a coat of HYB 021-03

Application 2

WB
HYB 021-08 recognizes human trypsin in Western blotting under reducing conditions.