互联网2013-11-13
The following protocol used formalin-fixed paraffin-embeddedhuman tonsil tissue (supplied by the Alfred Hospital, Australia).
1. Deparaffinize and rehydrate the tissue section.
2. Perform heat-induced antigen retrieval with 1mM EDTA pH 8.0 at 123?C for 3 minutes in a Pascal pressure chamber (Dako). Wash the slide twice for three minutes each (50mM Tris-HCl pH 8.0, 150mM NaCl in distilled water).
3. Incubate the tissue section with 1% BSA/PBS block.
4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each.
5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s protocol. Wash the slide twice for three minutes each.
6. Incubate the tissue section with the DAB chromogen.
7. Counterstain the tissue section with hematoxylin.
8. Coverslip the sections with DPX mounting medium and image sections with an Olympus Provis light microscope (Monash Micro Imaging, Monash University, Australia).
相关产品推荐
促销中二抗|HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L)
￥396￥440
Mouse Hprt qPCR Primer Pair，阿拉丁
￥29.90
Recombinant Human MCP1 Protein，阿拉丁
￥1099.90
Anti-Measles /MeV F/Fusion Antibody (mAb 77) 抗体
￥3868
Anti-MeV M/Matrix Polyclonal Antibody
相关问答
问
做ELISA检测人IgM时候，coat和酶标抗体可以都用goat anti human吗？
二抗anti-mouse可以代替anti-rat吗？
RABBIT ANTI HUMAN BETA AMYLOID (aa1-42)抗体做Elisa实验板的板包被，需要多少浓度的抗体
相关方法
细胞免疫化学（Immunohistochemistry）
2022-08-10
推荐阅读
Anti-Nitrotyrosine Antibodies for Immunohistochemistry
Human Anti-antibody Response
AbC Anti-Mouse Bead Kit
