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- BioPorto
- 丹麦
- CSI 003-23
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
1年
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 抗体英文名：
Anti-Vitronectin (human, dog, cat, goat, horse, bovine)
- 规格：
200ug
Anti-Vitronectin (human, dog, cat, goat, horse, bovine)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- CSI 003-23
- Product name
- Anti-Vitronectin (human, dog, cat, goat, horse, bovine)
- Description
- Mouse monoclonal antibody
- Specificity
-
CSI 003-23 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
CSI 003-23 binds nearly as well to native vitronectin as to denatured. CSI 003-23 is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.
- Immunogen
- Human vitronectin purified from plasma by heparin-affinity chromatography
- Application
- ELISA, WB
- Species reactivity (positive)
- Human, dog, cat, goat, bovine, horse
- Species reactivity (negative)
- Not determined
- Subclass
- IgG1/k
- Gene ID
- 7448, 101100765, 480621, 100861216, 507525, 100059034
- Clone number
- HV23
- Epitope
- Epitope is located in the somatomedin B domain
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (2).
- Application 1
-
ELISA
CSI 003-23 also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. In Western blotting a dilution guideline of 1/50 and 1/200 has proved successful (1,2). - Application 2
-
WB
CSI 003-23 can be used in Western blotting (1)
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
CAT ASSAY [TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks, shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml tubes.
a simple ELISA method to detect human anti-antibody response.
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