Anti-Antithrombin III (human,

Anti-Antithrombin III (human, native)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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Cat. No.

HYB 230-01

Product name

Anti-Antithrombin III (human, native)

Description

Mouse monoclonal antibody

Specificity

HYB 230-01 is specific for human Antithrombin III (AT III) native form

Immunogen

Antithrombin III isolated from human plasma

Application

ELISA

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

462

Clone number

1 B7

Epitope

Epitope specificity differs from that of HYB 230-04, HYB 228-10 and HYB 228-12.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components. 

Application 1

ELISA
HYB 230-01 reacts strongly with native AT III. Strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well. In a sandwich ELISA setup, HYB 230-01B acts as a detection antibody in conbination with ABS 014-22 as capture antibody to measure Thrombin-Antithrombin (TAT) complexes. 
 

The calibration curve of a sandwich assay for TAT (Thrombin-antithrombin) using ABS 014-22 as capture antibody and HYB 230-01B as the biotinylated detection antibody.