- ¥545
- BioPorto
- 丹麦
- CSI 001-76
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
1年
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 抗体英文名：
Anti-Perlecan (bovine, human)
- 规格：
200ug
Anti-Perlecan (bovine, human)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- CSI 001-76
- Product name
- Anti-Perlecan (bovine, human)
- Description
-
Mouse monoclonal antibody
- Specificity
-
CSI 001-76 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
- Immunogen
-
Lysed bovine corneal endothelial cells and extracellular matrix
- Application
- ELISA, WB, IHC-F, IHC-P, IP, AP
- Species reactivity (positive)
- Bovine, human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 444872, 3339
- Clone number
- A76
- Epitope
-
Not determined
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (1).
- Application 1
-
ELISA
CSI 001-76 can be used in ELISA. - Application 2
-
WB
CSI 001-76 can be used in Western blotting (1). - Application 3
-
IHC
CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. - Application 4
-
AP
CSI 001-76 is more sensitive than CSI 001-71 or CSI 001-74 in ELISA (2). - Application 5
-
IP
CSI 001-76 can be used in immunoprecipitation
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
a simple ELISA method to detect human anti-antibody response.
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
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